| POHANKA, M., Petr ZBOŘIL and J. PIKULA. D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography. Polish journal of chemistry. Warszawa: Państwowe Wydawnictwo Naukowe., 2009, vol. 83, No 3, p. 415-420. ISSN 0137-5083. |
Other formats:
BibTeX
LaTeX
RIS
@article{1674862,
author = {Pohanka, M. and Zbořil, Petr and Pikula, J.},
article_location = {Warszawa},
article_number = {3},
keywords = {baker's yeast; mitochondria; d-lactic acid; isolation},
language = {eng},
issn = {0137-5083},
journal = {Polish journal of chemistry},
title = {D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography},
url = {http://www.ichf.edu.pl/pjch/pj-2009.htm},
volume = {83},
year = {2009}
}
TY - JOUR
ID - 1674862
AU - Pohanka, M. - Zbořil, Petr - Pikula, J.
PY - 2009
TI - D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography
JF - Polish journal of chemistry
VL - 83
IS - 3
SP - 415-420
EP - 415-420
PB - Państwowe Wydawnictwo Naukowe.
SN - 01375083
KW - baker's yeast
KW - mitochondria
KW - d-lactic acid
KW - isolation
UR - http://www.ichf.edu.pl/pjch/pj-2009.htm
L2 - http://www.ichf.edu.pl/pjch/pj-2009.htm
N2 - D-Lactate dehydrogenase (cytochrome) was isolated from baker's yeasts (Saccharomyces cerevisiae) using fast protein liquid chromatography. Mitochondria from disrupted cells were separated and enzymes released by Triton X-100. Purification was performed by fast protein liquid chromatography on hydroxylapatite and in another separate experiment on AH-Sepharose with covalently bound 4-hydroxy-alpha cyanocinnamic acid (SHCA). Although purification on hydtoxylapatite provided approachable samples with activities for only D-lactic acid, chromatography on SHCA seemed to be better because a purified fraction of enzyme with the specific activity of 3.38 nkat/mg was obtained. Another problem considered was the usefulness of the combination of chromatography on hydroxylapatite and SHCA providing a fraction of D-lactic acid with specific activity of 5.71 nkat/mg and no cross reactivity for L-lactic acid.
ER -
POHANKA, M., Petr ZBOŘIL and J. PIKULA. D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography. \textit{Polish journal of chemistry}. Warszawa: Pa\'nstwowe Wydawnictwo Naukowe., 2009, vol.~83, No~3, p.~415-420. ISSN~0137-5083.
|
