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@article{1675476,
author = {Ješeta, Michal and Celá, Andrea and Žáková, Jana and Mádr, Aleš and Crha, Igor and Glatz, Zdeněk and Kempisty, Bartosz and Ventruba, Pavel},
article_location = {Basel},
article_number = {8},
doi = {http://dx.doi.org/10.3390/jcm9082609},
keywords = {amino acids; human embryo; in vitro cultivation; capillary electrophoresis; fluorescence detection; metabolic activity},
language = {eng},
issn = {2077-0383},
journal = {Journal of Clinical Medicine},
title = {Metabolic activity of human embryos after thawing differs in atmosphere with different oxygen concentrations},
url = {https://www.mdpi.com/2077-0383/9/8/2609},
volume = {9},
year = {2020}
}
TY - JOUR
ID - 1675476
AU - Ješeta, Michal - Celá, Andrea - Žáková, Jana - Mádr, Aleš - Crha, Igor - Glatz, Zdeněk - Kempisty, Bartosz - Ventruba, Pavel
PY - 2020
TI - Metabolic activity of human embryos after thawing differs in atmosphere with different oxygen concentrations
JF - Journal of Clinical Medicine
VL - 9
IS - 8
SP - 1-13
EP - 1-13
PB - MDPI
SN - 20770383
KW - amino acids
KW - human embryo
KW - in vitro cultivation
KW - capillary electrophoresis
KW - fluorescence detection
KW - metabolic activity
UR - https://www.mdpi.com/2077-0383/9/8/2609
L2 - https://www.mdpi.com/2077-0383/9/8/2609
N2 - The vitrification of human embryos is more and more frequently being utilized as a method of assisted reproduction. For this technique, gentle treatment of the embryos after thawing is crucial. In this study, the balance of amino acids released to/consumed from the cultivation media surrounding the warmed embryos was observed in the context of a cultivation environment, which was with the atmospheric oxygen concentration approximate to 20% or with a regulated oxygen level-hysiological (5%). It is the first time that total amino acid turnover in human embryos after their freezing at post compaction stages has been evaluated. During this study, progressive embryos (developed to blastocyst stage) and stagnant embryos (without developmental progression) were analyzed. It was observed that the embryos cultivated in conditions of physiological oxygen levels (5% oxygen) showed a significantly lower consumption of amino acids from the cultivation media. Progressively developing embryos also had significantly lower total amino acid turnovers (consumption and production of amino acids) when cultured in conditions with physiological oxygen levels. Based on these results it seems that a cultivation environment with a reduced oxygen concentration decreases the risk of degenerative changes in the embryos after thawing. Therefore, the cultivation of thawed embryos in an environment with physiological oxygen levels may preclude embryonal stagnation, and can support the further development of human embryos after their thawing.
ER -
JEŠETA, Michal, Andrea CELÁ, Jana ŽÁKOVÁ, Aleš MÁDR, Igor CRHA, Zdeněk GLATZ, Bartosz KEMPISTY a Pavel VENTRUBA. Metabolic activity of human embryos after thawing differs in atmosphere with different oxygen concentrations. \textit{Journal of Clinical Medicine}. Basel: MDPI, roč.~9, č.~8, s.~1-13. ISSN~2077-0383. doi:10.3390/jcm9082609. 2020.
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