JEŠETA, Michal, Andrea CELÁ, Jana ŽÁKOVÁ, Aleš MÁDR, Igor CRHA, Zdeněk GLATZ, Bartosz KEMPISTY and Pavel VENTRUBA. Metabolic activity of human embryos after thawing differs in atmosphere with different oxygen concentrations. Journal of Clinical Medicine. Basel: MDPI, 2020, vol. 9, No 8, p. 1-13. ISSN 2077-0383. doi:10.3390/jcm9082609.
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Basic information
Original name Metabolic activity of human embryos after thawing differs in atmosphere with different oxygen concentrations
Name in Czech Metabolická aktivita lidských embryí po rozmrazení se liší v atmosféře s různými koncentracemi kyslíku
Authors JEŠETA, Michal (203 Czech Republic, guarantor, belonging to the institution), Andrea CELÁ (203 Czech Republic, belonging to the institution), Jana ŽÁKOVÁ (203 Czech Republic, belonging to the institution), Aleš MÁDR (203 Czech Republic, belonging to the institution), Igor CRHA (203 Czech Republic, belonging to the institution), Zdeněk GLATZ (203 Czech Republic, belonging to the institution), Bartosz KEMPISTY (616 Poland) and Pavel VENTRUBA (203 Czech Republic, belonging to the institution).
Edition Journal of Clinical Medicine, Basel, MDPI, 2020, 2077-0383.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30218 General and internal medicine
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.303 in 2019
RIV identification code RIV/00216224:14110/20:00116273
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.3390/jcm9082609
UT WoS 000568129200001
Keywords (in Czech) aminokyseliny; lidské embryo; kultivace in vitro; kapilární elektroforéza; fluorescenční detekce; metabolická aktivita
Keywords in English amino acids; human embryo; in vitro cultivation; capillary electrophoresis; fluorescence detection; metabolic activity
Tags 14110411, 14110611, GPK, GPKgyn, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 23. 2. 2021 14:02.
Abstract
The vitrification of human embryos is more and more frequently being utilized as a method of assisted reproduction. For this technique, gentle treatment of the embryos after thawing is crucial. In this study, the balance of amino acids released to/consumed from the cultivation media surrounding the warmed embryos was observed in the context of a cultivation environment, which was with the atmospheric oxygen concentration approximate to 20% or with a regulated oxygen level-hysiological (5%). It is the first time that total amino acid turnover in human embryos after their freezing at post compaction stages has been evaluated. During this study, progressive embryos (developed to blastocyst stage) and stagnant embryos (without developmental progression) were analyzed. It was observed that the embryos cultivated in conditions of physiological oxygen levels (5% oxygen) showed a significantly lower consumption of amino acids from the cultivation media. Progressively developing embryos also had significantly lower total amino acid turnovers (consumption and production of amino acids) when cultured in conditions with physiological oxygen levels. Based on these results it seems that a cultivation environment with a reduced oxygen concentration decreases the risk of degenerative changes in the embryos after thawing. Therefore, the cultivation of thawed embryos in an environment with physiological oxygen levels may preclude embryonal stagnation, and can support the further development of human embryos after their thawing.
Links
MUNI/A/1252/2019, internal MU codeName: Podpora biochemického výzkumu v roce 2020
Investor: Masaryk University, Grant Agency of Masaryk University, Category A
NV18-08-00412, research and development projectName: Lidské embryonální kmenové buňky pro klinické aplikace: derivace a charakterizace
Investor: Ministry of Health of the CR, Ministry of Health Research Programme 2015 - 2022
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