k 2020

Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry

VAŇHARA, Petr, Andreas SCHNAPP, Lukáš MORÁŇ, Lukáš PEČINKA, Hana KOTASOVÁ et. al.

Basic information

Original name

Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry

Authors

Edition

68th Annual Conference of American Society for Mass Spectrometry, Houston, TX, USA, 2020

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Keywords (in Czech)

embryonální kmenové buňky; hmotnostní spektrometrie; monitoring; diferenciace

Keywords in English

embryonic stem cells; mass spectrometry; monitoring; differentiation

Tags

International impact, Reviewed
Změněno: 9/10/2020 14:13, Mgr. Lukáš Pečinka, Ph.D.

Abstract

V originále

Human embryonic stem cells (hESCs) represent a promising tool for clinical applications. hESCs differentiation to relevant cell types is a gradual process, where final phenotype is achieved. However, substantial heterogeneity in differentiation process may produce aberrant cells with unwanted properties, such as lack of functional phenotype, or propensity to cancer growth. Early lung progenitors (ELEPs) are direct precursors of mature lung cells (pneumocytes) that differentiate from hESCs under specific conditions through several stages. Differentiation pathway can be outlined specifically by molecular markers, but an unbiased, sensitive and robust tool for discrimination of ELEPs from pluripotent or transitional stages is still missing. Here we demonstrate that intact cell MALDI TOF mass spectrometry may provide such a tool for quality control.