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@inbook{1675802,
author = {Schořová, Šárka and Fajkus, Jiří and Procházková Schrumpfová, Petra},
address = {New York, USA},
booktitle = {The Nucleus - Methods in Molecular Biology},
doi = {http://dx.doi.org/10.1007/978-1-0716-0763-3_11},
editor = {Ronald Hancock},
keywords = {Methods; protein-protein interaction; DNA-protein interaction; Bimolecular fluorescence complementation; Co-immunoprecipitation; Yeast two-hybrid system; ChIP for low-abundance proteins},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York, USA},
isbn = {978-1-0716-0762-6},
pages = {139-167},
publisher = {Springer Nature, Humana Press},
title = {Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres},
url = {https://doi.org/10.1007/978-1-0716-0763-3_11},
year = {2020}
}
TY - CHAP
ID - 1675802
AU - Schořová, Šárka - Fajkus, Jiří - Procházková Schrumpfová, Petra
PY - 2020
TI - Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres
VL - Third Edition
PB - Springer Nature, Humana Press
CY - New York, USA
SN - 9781071607626
KW - Methods
KW - protein-protein interaction
KW - DNA-protein interaction
KW - Bimolecular fluorescence complementation
KW - Co-immunoprecipitation
KW - Yeast two-hybrid system
KW - ChIP for low-abundance proteins
UR - https://doi.org/10.1007/978-1-0716-0763-3_11
N2 - Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP), yeast two-hybrid systems (Y2H), and chromatin immunoprecipitation (ChIP). These improved protocols detect trimeric complexes in which two proteins of interest interact indirectly via a protein sandwiched between them. They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. Here we describe these methods and their application to map interactions of several telomere- and telomerase-associated proteins and to purify a sufficient amount of chromatin from Arabidopsis thaliana for further investigations (e.g., next-generation sequencing, hybridization)
ER -
SCHOŘOVÁ, Šárka, Jiří FAJKUS and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres. In Ronald Hancock. \textit{The Nucleus - Methods in Molecular Biology}. New York, USA: Springer Nature, Humana Press, 2020, p.~139-167. Third Edition. ISBN~978-1-0716-0762-6. Available from: https://dx.doi.org/10.1007/978-1-0716-0763-3\_{}11.
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