Optimized Detection of Protein-Protein and Protein-DNA
Interactions, with Particular Application to Plant Telomeres

C 2020

Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres

SCHOŘOVÁ, Šárka, Jiří FAJKUS and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ

Basic information

Original name

Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres

Authors

SCHOŘOVÁ, Šárka (203 Czech Republic, belonging to the institution), Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution) and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ (203 Czech Republic, belonging to the institution)

Edition

New York, USA, The Nucleus - Methods in Molecular Biology, p. 139-167, 29 pp. Third Edition, 2020

Publisher

Springer Nature, Humana Press

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

RIV identification code

RIV/00216224:14310/20:00114288

Organization unit

Faculty of Science

ISBN

978-1-0716-0762-6

DOI

http://dx.doi.org/10.1007/978-1-0716-0763-3_11

UT WoS

000681366300012

Keywords in English

Methods; protein-protein interaction; DNA-protein interaction; Bimolecular fluorescence complementation; Co-immunoprecipitation; Yeast two-hybrid system; ChIP for low-abundance proteins

Abstract

V originále

Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP), yeast two-hybrid systems (Y2H), and chromatin immunoprecipitation (ChIP). These improved protocols detect trimeric complexes in which two proteins of interest interact indirectly via a protein sandwiched between them. They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. Here we describe these methods and their application to map interactions of several telomere- and telomerase-associated proteins and to purify a sufficient amount of chromatin from Arabidopsis thaliana for further investigations (e.g., next-generation sequencing, hybridization)

Links

GA17-09644S, research and development project

Name: Molekulární podstata evolučních přeměn telomer u rostlin řádu Asparagales

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SCHOŘOVÁ, Šárka, Jiří FAJKUS and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres. In Ronald Hancock. The Nucleus - Methods in Molecular Biology. New York, USA: Springer Nature, Humana Press, 2020, p. 139-167. Third Edition. ISBN 978-1-0716-0762-6. Available from: https://dx.doi.org/10.1007/978-1-0716-0763-3_11.

@inbook{1675802,
   author = {Schořová, Šárka and Fajkus, Jiří and Procházková Schrumpfová, Petra},
   address = {New York, USA},
   booktitle = {The Nucleus - Methods in Molecular Biology},
   doi = {http://dx.doi.org/10.1007/978-1-0716-0763-3_11},
   editor = {Ronald Hancock},
   keywords = {Methods; protein-protein interaction; DNA-protein interaction; Bimolecular fluorescence complementation; Co-immunoprecipitation; Yeast two-hybrid system; ChIP for low-abundance proteins},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York, USA},
   isbn = {978-1-0716-0762-6},
   pages = {139-167},
   publisher = {Springer Nature, Humana Press},
   title = {Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres},
   url = {https://doi.org/10.1007/978-1-0716-0763-3_11},
   year = {2020}
}

TY  - CHAP
ID  - 1675802
AU  - Schořová, Šárka - Fajkus, Jiří - Procházková Schrumpfová, Petra
PY  - 2020
TI  - Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres
VL  - Third Edition
PB  - Springer Nature, Humana Press
CY  - New York, USA
SN  - 9781071607626
KW  - Methods
KW  - protein-protein interaction
KW  - DNA-protein interaction
KW  - Bimolecular fluorescence complementation
KW  - Co-immunoprecipitation
KW  - Yeast two-hybrid system
KW  - ChIP for low-abundance proteins
UR  - https://doi.org/10.1007/978-1-0716-0763-3_11
N2  - Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP), yeast two-hybrid systems (Y2H), and chromatin immunoprecipitation (ChIP). These improved protocols detect trimeric complexes in which two proteins of interest interact indirectly via a protein sandwiched between them. They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. Here we describe these methods and their application to map interactions of several telomere- and telomerase-associated proteins and to purify a sufficient amount of chromatin from Arabidopsis thaliana for further investigations (e.g., next-generation sequencing, hybridization)
ER  -

SCHOŘOVÁ, Šárka, Jiří FAJKUS and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres. In Ronald Hancock. \textit{The Nucleus - Methods in Molecular Biology}. New York, USA: Springer Nature, Humana Press, 2020, p.~139-167. Third Edition. ISBN~978-1-0716-0762-6. Available from: https://dx.doi.org/10.1007/978-1-0716-0763-3\_{}11.

Detailed Information on Publication Record