SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress

NAGY, Livia, Tuende ANGYAL, Geza NAGY, Matsumoto AKIKO, Jan PŘIBYL and Petr SKLÁDAL. SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress. Online. In Vieira, P.; Fred, A.; Filipe, J.; Gamboa, H. BIODEVICES 2011. SETUBAL: SCITEPRESS, 2011, p. 359-362. ISBN 978-989-8425-37-9.

Basic information

• Original name: SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress
• Authors: NAGY, Livia, Tuende ANGYAL, Geza NAGY, Matsumoto AKIKO, Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, belonging to the institution).
• Edition: SETUBAL, BIODEVICES 2011, p. 359-362, 4 pp. 2011.
• Publisher: SCITEPRESS

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 20602 Medical laboratory technology
• Country of publisher: Portugal
• Confidentiality degree: is not subject to a state or trade secret
• Publication form: electronic version available online
• WWW: URL
• Organization unit: Faculty of Science
• ISBN: 978-989-8425-37-9
• UT WoS: 000308513400068
• Keywords in English: Alcohol induced oxidative stress; Reactive oxygen species (ROS); and Electrochemical detection
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

The role of reactive oxidizing species (ROS) is proved within numerous physiological processes, including aging, signal transduction and some kind of immune functions. Nowadays ROS and oxidative stress gain increasing attention in connection with a wide spectrum of diseases. In case of Asian people, the enzyme taking part in the ethanol metabolism, the aldehyde dehydrogenase is absent or mutated, that can result in liver tissue damage upon extensive alcohol consumption. Most of the ROS species are electrochemically active therefore the applications of electrochemical methods are the most promising for in situ or in vivo monitoring or quantification of them. In our work development and improvements of selective and sensitive method for electrochemical detection of these molecules and radicals are attempted. We prepared ultra thin size-exclusion layer by electropolymerization of m-phenylenediamine monomer on the surface of the Pt working electrode to ensure its selectivity. We have worked out the optimal circumstances for the selective layer preparation and tested its stability and function. In order to enhance the sensitivity of ROS detection a new amperometric method, the periodically interrupted amperometry (PIA) was developed and applied. With this approach we succeeded selective and sensitive detection of H2O2 in vitro.