Direct fluorogenic detection of palladium and platinum
organometallic complexes with proteins and nucleic acids in
polyacrylamide gels

J 2020

Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels

PEKAŘÍK, Vladimír, Marie KUDLIČKOVÁ PEŠKOVÁ, Jakub DUBEN, Marek REMES, Zbynek HEGER et. al.

Basic information

Original name

Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels

Authors

PEKAŘÍK, Vladimír (203 Czech Republic, guarantor, belonging to the institution), Marie KUDLIČKOVÁ PEŠKOVÁ (203 Czech Republic, belonging to the institution), Jakub DUBEN (703 Slovakia, belonging to the institution), Marek REMES (203 Czech Republic) and Zbynek HEGER (203 Czech Republic)

Edition

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2020, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.379

RIV identification code

RIV/00216224:14110/20:00116305

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-020-69336-w

UT WoS

000556690900051

Keywords in English

Direct fluorogenic detection; palladium and platinum organometallic complexes; polyacrylamide gels

Abstract

V originále

Allyl- and propargyl ethers of umbelliferone are sensitive probes for palladium and platinum, including anticancer compounds cisplatin, carboplatin and oxaliplatin, and effective for direct visualization of protein and DNA complexes with organometallic compounds in polyacrylamide gels allowing easy detection of interactions with analyzed protein or nucleic acid. Both probes can be used for fast evaluation of Pd/Pt binding to nanocarriers relevant in drug targeted therapy or specific clinically relevant target macromolecules.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90043, large research infrastructures

Name: CIISB

Citovat

PEKAŘÍK, Vladimír, Marie KUDLIČKOVÁ PEŠKOVÁ, Jakub DUBEN, Marek REMES and Zbynek HEGER. Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2020, vol. 10, No 1, p. 1-10. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-69336-w.

@article{1676538,
   author = {Pekařík, Vladimír and Kudličková Pešková, Marie and Duben, Jakub and Remes, Marek and Heger, Zbynek},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-020-69336-w},
   keywords = {Direct fluorogenic detection; palladium and platinum organometallic complexes; polyacrylamide gels},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels},
   url = {https://www.nature.com/articles/s41598-020-69336-w.pdf},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1676538
AU  - Pekařík, Vladimír - Kudličková Pešková, Marie - Duben, Jakub - Remes, Marek - Heger, Zbynek
PY  - 2020
TI  - Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
JF  - Scientific reports
VL  - 10
IS  - 1
SP  - 1-10
EP  - 1-10
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - Direct fluorogenic detection
KW  - palladium and platinum organometallic complexes
KW  - polyacrylamide gels
UR  - https://www.nature.com/articles/s41598-020-69336-w.pdf
L2  - https://www.nature.com/articles/s41598-020-69336-w.pdf
N2  - Allyl- and propargyl ethers of umbelliferone are sensitive probes for palladium and platinum, including anticancer compounds cisplatin, carboplatin and oxaliplatin, and effective for direct visualization of protein and DNA complexes with organometallic compounds in polyacrylamide gels allowing easy detection of interactions with analyzed protein or nucleic acid. Both probes can be used for fast evaluation of Pd/Pt binding to nanocarriers relevant in drug targeted therapy or specific clinically relevant target macromolecules.
ER  -

PEKAŘÍK, Vladimír, Marie KUDLIČKOVÁ PEŠKOVÁ, Jakub DUBEN, Marek REMES and Zbynek HEGER. Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2020, vol.~10, No~1, p.~1-10. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-69336-w.

Detailed Information on Publication Record