Senescence-like phenotype in post-mitotic cells of mice
entering middle age

J 2020

Senescence-like phenotype in post-mitotic cells of mice entering middle age

RAFFAELE, Marco, Kristína KOVAČOVICOVÁ, Francesca BONOMINI, Rita REZZANI, Jan FROHLICH et. al.

Basic information

Original name

Senescence-like phenotype in post-mitotic cells of mice entering middle age

Authors

RAFFAELE, Marco (380 Italy), Kristína KOVAČOVICOVÁ (203 Czech Republic), Francesca BONOMINI (380 Italy), Rita REZZANI (380 Italy), Jan FROHLICH (203 Czech Republic) and Manlio VINCIGUERRA (380 Italy, guarantor, belonging to the institution)

Edition

AGING-US, ORCHARD PARK, IMPACT JOURNALS LLC, 2020, 1945-4589

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.682

RIV identification code

RIV/00216224:14110/20:00116306

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.18632/aging.103637

UT WoS

000558672300001

Keywords in English

senescence; mice; markers

Abstract

V originále

Staining mice tissues for beta-galactosidase activity is a fundamental tool to detect age- or disease-associated cellular senescence. However, reported analyses of positivity for senescence-associated beta-galactosidase activity or for other markers of senescence in post-mitotic cells of healthy murine tissues have been fragmentary or inconclusive. Here, we attempted to independently deepen this knowledge using multiple senescence markers within the same cells of wild type mice entering middle age (9 months of age). A histochemistry protocol for the pH-dependent detection of beta-galactosidase activity in several tissues was used. At pH 6, routinely utilized to detect senescence-associated beta-galactosidase activity, only specific cellular populations in the mouse body (including Purkinje cells and choroid plexus in the central nervous system) were detected as strongly positive for beta-galactosidase activity. These post-mitotic cells were also positive for other established markers of senescence (p16, p21 and DPP4), detected by immunofluorescence, confirming a potential senescent phenotype. These data might contribute to understanding the functional relation between the senescence-associated beta-galactosidase activity and senescence markers in post-mitotic cells in absence of disease or advanced aging.

Citovat

RAFFAELE, Marco, Kristína KOVAČOVICOVÁ, Francesca BONOMINI, Rita REZZANI, Jan FROHLICH and Manlio VINCIGUERRA. Senescence-like phenotype in post-mitotic cells of mice entering middle age. AGING-US. ORCHARD PARK: IMPACT JOURNALS LLC, 2020, vol. 12, No 14, p. 13979-13990. ISSN 1945-4589. Available from: https://dx.doi.org/10.18632/aging.103637.

@article{1676539,
   author = {Raffaele, Marco and Kovačovicová, Kristína and Bonomini, Francesca and Rezzani, Rita and Frohlich, Jan and Vinciguerra, Manlio},
   article_location = {ORCHARD PARK},
   article_number = {14},
   doi = {http://dx.doi.org/10.18632/aging.103637},
   keywords = {senescence; mice; markers},
   language = {eng},
   issn = {1945-4589},
   journal = {AGING-US},
   title = {Senescence-like phenotype in post-mitotic cells of mice entering middle age},
   url = {https://www.aging-us.com/article/103637},
   volume = {12},
   year = {2020}
}

TY  - JOUR
ID  - 1676539
AU  - Raffaele, Marco - Kovačovicová, Kristína - Bonomini, Francesca - Rezzani, Rita - Frohlich, Jan - Vinciguerra, Manlio
PY  - 2020
TI  - Senescence-like phenotype in post-mitotic cells of mice entering middle age
JF  - AGING-US
VL  - 12
IS  - 14
SP  - 13979-13990
EP  - 13979-13990
PB  - IMPACT JOURNALS LLC
SN  - 19454589
KW  - senescence
KW  - mice
KW  - markers
UR  - https://www.aging-us.com/article/103637
L2  - https://www.aging-us.com/article/103637
N2  - Staining mice tissues for beta-galactosidase activity is a fundamental tool to detect age- or disease-associated cellular senescence. However, reported analyses of positivity for senescence-associated beta-galactosidase activity or for other markers of senescence in post-mitotic cells of healthy murine tissues have been fragmentary or inconclusive. Here, we attempted to independently deepen this knowledge using multiple senescence markers within the same cells of wild type mice entering middle age (9 months of age). A histochemistry protocol for the pH-dependent detection of beta-galactosidase activity in several tissues was used. At pH 6, routinely utilized to detect senescence-associated beta-galactosidase activity, only specific cellular populations in the mouse body (including Purkinje cells and choroid plexus in the central nervous system) were detected as strongly positive for beta-galactosidase activity. These post-mitotic cells were also positive for other established markers of senescence (p16, p21 and DPP4), detected by immunofluorescence, confirming a potential senescent phenotype. These data might contribute to understanding the functional relation between the senescence-associated beta-galactosidase activity and senescence markers in post-mitotic cells in absence of disease or advanced aging.
ER  -

RAFFAELE, Marco, Kristína KOVAČOVICOVÁ, Francesca BONOMINI, Rita REZZANI, Jan FROHLICH and Manlio VINCIGUERRA. Senescence-like phenotype in post-mitotic cells of mice entering middle age. \textit{AGING-US}. ORCHARD PARK: IMPACT JOURNALS LLC, 2020, vol.~12, No~14, p.~13979-13990. ISSN~1945-4589. Available from: https://dx.doi.org/10.18632/aging.103637.

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