JOUKAL, Marek, Lucy VULCHANOVA, Cecilia HUFFMAN, Petr DUBOVÝ and Christopher N. HONDA. Peripheral Deltorphin II Inhibits Nociceptors Following Nerve Injury. Frontiers in Pharmacology. Lausanne: Frontiers Media SA, 2020, vol. 11, JUL 2020, p. 1-10. ISSN 1663-9812. Available from: https://dx.doi.org/10.3389/fphar.2020.01151.
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Basic information
Original name Peripheral Deltorphin II Inhibits Nociceptors Following Nerve Injury
Authors JOUKAL, Marek (203 Czech Republic, belonging to the institution), Lucy VULCHANOVA (840 United States of America), Cecilia HUFFMAN (840 United States of America), Petr DUBOVÝ (203 Czech Republic, belonging to the institution) and Christopher N. HONDA (840 United States of America, guarantor).
Edition Frontiers in Pharmacology, Lausanne, Frontiers Media SA, 2020, 1663-9812.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30104 Pharmacology and pharmacy
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.810
RIV identification code RIV/00216224:14110/20:00116347
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.3389/fphar.2020.01151
UT WoS 000561956900001
Keywords in English nociceptors; spared nerve injury; delta opioid receptors; neuropathic pain; deltorphin II
Tags 14110514, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 12/5/2021 13:45.
Abstract
Clinical and preclinical studies have revealed that local administration of opioid agonists into peripheral tissue attenuates inflammatory pain. However, few studies have examined whether peripherally restricted opioids are effective in reducing mechanical allodynia and hyperalgesia that usually follows nerve injury. The aim of the present study was to determine whether the mechanical responsiveness of C-fiber mechanical nociceptors innervating skin under neuropathic pain conditions is depressed by direct activation of delta opioid receptors (DORs) on their peripheral terminals. A murine model of peripheral neuropathic pain was induced with a spared nerve (tibial) injury, in which mice survived 7 or 28 days after surgery before electrophysiological testing began. Control groups comprised naive and sham-operated animals. An ex vivo preparation of mouse plantar skin with attached tibial nerve was used to examine electrophysiologically the effects of the selective DOR agonist, deltorphin II, on the response properties of individual cutaneous C-fiber nociceptors. In contrast to naive and sham-operated animals, deltorphin II induced an inhibition of the mechanical responsiveness of C-fiber mechanical nociceptors innervating skin under neuropathic conditions. The effects of deltorphin II were concentration-dependent and prevented by pretreatment with naltrindole indicating DOR-mediated inhibitory effects of deltorphin II. Our results provide the first direct evidence for expression of functional DORs on mechanical nociceptors innervating skin in an animal model of neuropathic pain.
Links
ROZV/23/LF14/2019, interní kód MUName: Elektrofyziologická a imunohistochemická studie změn podmíněných neurozánětem ve spinálních gangliích neasociovaných s poškozeným periferním nervem
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
ROZV/24/LF/2018, interní kód MUName: LF - Příspěvek na IP 2108
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
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