Cortical Granule Distribution and Expression Pattern of Genes
Regulating Cellular Component Size, Morphogenesis, and
Potential to Differentiation are Related to Oocyte
Developmental Competence and Maturational Capacity In Vivo and
In Vitro

J 2020

Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro

KULUS, M., W. KRANC, Michal JEŠETA, P. SUJKA-KORDOWSKA, A. KONWERSKA et. al.

Basic information

Original name

Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro

Authors

KULUS, M. (616 Poland), W. KRANC (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), P. SUJKA-KORDOWSKA (616 Poland), A. KONWERSKA (616 Poland), S. CIESIOLKA (616 Poland), P. CELICHOWSKI (616 Poland), L. MONCRIEFF (826 United Kingdom of Great Britain and Northern Ireland), I. KOCHEROVA (616 Poland), M. JOZKOWIAK (616 Poland), J. KULUS (616 Poland), M. WIECZORKIEWICZ (616 Poland), H. PIOTROWSKA-KEMPISTY (616 Poland), M. T. SKOWRONSKI (616 Poland), D. BUKOWSKA (616 Poland), M. MACHATKOVA (203 Czech Republic), S. HANULAKOVA (203 Czech Republic), P. MOZDZIAK (840 United States of America), J. M. JASKOWSKI (616 Poland), B. KEMPISTY (616 Poland, guarantor) and P. ANTOSIK (616 Poland)

Edition

Genes, Basilej, MDPI, 2020, 2073-4425

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.096

RIV identification code

RIV/00216224:14110/20:00116349

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/genes11070815

UT WoS

000557982300001

Keywords in English

pig; oocyte maturation; microarray; cortical granule; molecular markers

Abstract

V originále

Polyspermia is an adverse phenomenon during mammalian fertilization when more than one sperm fuses with a single oocyte. The egg cell is prepared to prevent polyspermia by, among other ways, producing cortical granules (CGs), which are specialized intracellular structures containing enzymes that aim to harden the zona pellucida and block the fusion of subsequent sperm. This work focused on exploring the expression profile of genes that may be associated with cortical reactions, and evaluated the distribution of CGs in immature oocytes and the peripheral density of CGs in mature oocytes. Oocytes were isolated and then processed for in vitro maturation (IVM). Transcriptomic analysis of genes belonging to five ontological groups has been conducted. Six genes showed increased expression after IVM (ARHGEF2,MAP1B,CXCL12,FN1,DAB2, andSOX9), while the majority of genes decreased expression after IVM. Using CG distribution analysis in immature oocytes, movement towards the cortical zone of the oocyte during meiotic competence acquisition was observed. CGs peripheral density decreased with the rise in meiotic competence during the IVM process. The current results reveal important new insights into the in vitro maturation of oocytes. Our results may serve as a basis for further studies to investigate the cortical reaction of oocytes.

Citovat

KULUS, M., W. KRANC, Michal JEŠETA, P. SUJKA-KORDOWSKA, A. KONWERSKA, S. CIESIOLKA, P. CELICHOWSKI, L. MONCRIEFF, I. KOCHEROVA, M. JOZKOWIAK, J. KULUS, M. WIECZORKIEWICZ, H. PIOTROWSKA-KEMPISTY, M. T. SKOWRONSKI, D. BUKOWSKA, M. MACHATKOVA, S. HANULAKOVA, P. MOZDZIAK, J. M. JASKOWSKI, B. KEMPISTY and P. ANTOSIK. Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro. Genes. Basilej: MDPI, 2020, vol. 11, No 7, p. 1-27. ISSN 2073-4425. Available from: https://dx.doi.org/10.3390/genes11070815.

@article{1677685,
   author = {Kulus, M. and Kranc, W. and Ješeta, Michal and SujkaandKordowska, P. and Konwerska, A. and Ciesiolka, S. and Celichowski, P. and Moncrieff, L. and Kocherova, I. and Jozkowiak, M. and Kulus, J. and Wieczorkiewicz, M. and PiotrowskaandKempisty, H. and Skowronski, M. T. and Bukowska, D. and Machatkova, M. and Hanulakova, S. and Mozdziak, P. and Jaskowski, J. M. and Kempisty, B. and Antosik, P.},
   article_location = {Basilej},
   article_number = {7},
   doi = {http://dx.doi.org/10.3390/genes11070815},
   keywords = {pig; oocyte maturation; microarray; cortical granule; molecular markers},
   language = {eng},
   issn = {2073-4425},
   journal = {Genes},
   title = {Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro},
   url = {https://www.mdpi.com/2073-4425/11/7/815},
   volume = {11},
   year = {2020}
}

TY  - JOUR
ID  - 1677685
AU  - Kulus, M. - Kranc, W. - Ješeta, Michal - Sujka-Kordowska, P. - Konwerska, A. - Ciesiolka, S. - Celichowski, P. - Moncrieff, L. - Kocherova, I. - Jozkowiak, M. - Kulus, J. - Wieczorkiewicz, M. - Piotrowska-Kempisty, H. - Skowronski, M. T. - Bukowska, D. - Machatkova, M. - Hanulakova, S. - Mozdziak, P. - Jaskowski, J. M. - Kempisty, B. - Antosik, P.
PY  - 2020
TI  - Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro
JF  - Genes
VL  - 11
IS  - 7
SP  - 1-27
EP  - 1-27
PB  - MDPI
SN  - 20734425
KW  - pig
KW  - oocyte maturation
KW  - microarray
KW  - cortical granule
KW  - molecular markers
UR  - https://www.mdpi.com/2073-4425/11/7/815
L2  - https://www.mdpi.com/2073-4425/11/7/815
N2  - Polyspermia is an adverse phenomenon during mammalian fertilization when more than one sperm fuses with a single oocyte. The egg cell is prepared to prevent polyspermia by, among other ways, producing cortical granules (CGs), which are specialized intracellular structures containing enzymes that aim to harden the zona pellucida and block the fusion of subsequent sperm. This work focused on exploring the expression profile of genes that may be associated with cortical reactions, and evaluated the distribution of CGs in immature oocytes and the peripheral density of CGs in mature oocytes. Oocytes were isolated and then processed for in vitro maturation (IVM). Transcriptomic analysis of genes belonging to five ontological groups has been conducted. Six genes showed increased expression after IVM (ARHGEF2,MAP1B,CXCL12,FN1,DAB2, andSOX9), while the majority of genes decreased expression after IVM. Using CG distribution analysis in immature oocytes, movement towards the cortical zone of the oocyte during meiotic competence acquisition was observed. CGs peripheral density decreased with the rise in meiotic competence during the IVM process. The current results reveal important new insights into the in vitro maturation of oocytes. Our results may serve as a basis for further studies to investigate the cortical reaction of oocytes.
ER  -

KULUS, M., W. KRANC, Michal JEŠETA, P. SUJKA-KORDOWSKA, A. KONWERSKA, S. CIESIOLKA, P. CELICHOWSKI, L. MONCRIEFF, I. KOCHEROVA, M. JOZKOWIAK, J. KULUS, M. WIECZORKIEWICZ, H. PIOTROWSKA-KEMPISTY, M. T. SKOWRONSKI, D. BUKOWSKA, M. MACHATKOVA, S. HANULAKOVA, P. MOZDZIAK, J. M. JASKOWSKI, B. KEMPISTY and P. ANTOSIK. Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro. \textit{Genes}. Basilej: MDPI, 2020, vol.~11, No~7, p.~1-27. ISSN~2073-4425. Available from: https://dx.doi.org/10.3390/genes11070815.

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