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@article{1677829,
author = {Simeckova, Pavlina and Hubatka, Frantisek and Kotoucek, Jan and Knotigova Turanek, Pavlina and Masek, Josef and Slavik, Josef and Kovac, Ondrej and Neca, Jiri and Kulich, Pavel and Hrebík, Dominik and Straska, Jana and Pencikova, Katerina and Prochazkova, Jirina and Divis, Pavel and Macaulay, Stuart and Mikulík, Robert and Raska, Milan and Machala, Miroslav and Turánek, Jaroslav},
article_location = {LONDON},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-020-60284-z},
keywords = {HEPARG CELLS; MOLECULAR-MECHANISMS; OXIDATIVE STRESS; GENE-EXPRESSION; CONTRAST AGENTS; DTPA LIPOSOMES; TOXICITY; GRAPHENE; DISEASES; ENZYMES},
language = {eng},
issn = {2045-2322},
journal = {Scientific reports},
title = {Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages},
url = {https://www.nature.com/articles/s41598-020-60284-z.pdf},
volume = {10},
year = {2020}
}
TY - JOUR
ID - 1677829
AU - Simeckova, Pavlina - Hubatka, Frantisek - Kotoucek, Jan - Knotigova Turanek, Pavlina - Masek, Josef - Slavik, Josef - Kovac, Ondrej - Neca, Jiri - Kulich, Pavel - Hrebík, Dominik - Straska, Jana - Pencikova, Katerina - Prochazkova, Jirina - Divis, Pavel - Macaulay, Stuart - Mikulík, Robert - Raska, Milan - Machala, Miroslav - Turánek, Jaroslav
PY - 2020
TI - Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages
JF - Scientific reports
VL - 10
IS - 1
SP - 1-13
EP - 1-13
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - HEPARG CELLS
KW - MOLECULAR-MECHANISMS
KW - OXIDATIVE STRESS
KW - GENE-EXPRESSION
KW - CONTRAST AGENTS
KW - DTPA LIPOSOMES
KW - TOXICITY
KW - GRAPHENE
KW - DISEASES
KW - ENZYMES
UR - https://www.nature.com/articles/s41598-020-60284-z.pdf
L2 - https://www.nature.com/articles/s41598-020-60284-z.pdf
N2 - Gadolinium (Gd)-based contrast agents are extensively used for magnetic resonance imaging (MRI). Liposomes are potential nanocarrier-based biocompatible platforms for development of new generations of MRI diagnostics. Liposomes with Gd-complexes (Gd-lip) co-encapsulated with thrombolytic agents can serve both for imaging and treatment of various pathological states including stroke. In this study, we evaluated nanosafety of Gd-lip containing PE-DTPA chelating Gd+3 prepared by lipid film hydration method. We detected no cytotoxicity of Gd-lip in human liver cells including cancer HepG2, progenitor (non-differentiated) HepaRG, and differentiated HepaRG cells. Furthermore, no potential side effects of Gd-lip were found using a complex system including general biomarkers of toxicity, such as induction of early response genes, oxidative, heat shock and endoplasmic reticulum stress, DNA damage responses, induction of xenobiotic metabolizing enzymes, and changes in sphingolipid metabolism in differentiated HepaRG. Moreover, Gd-lip did not show pro-inflammatory effects, as assessed in an assay based on activation of inflammasome NLRP3 in a model of human macrophages, and release of eicosanoids from HepaRG cells. In conclusion, this in vitro study indicates potential in vivo safety of Gd-lip with respect to hepatotoxicity and immunopathology caused by inflammation.
ER -
SIMECKOVA, Pavlina, Frantisek HUBATKA, Jan KOTOUCEK, Pavlina KNOTIGOVA TURANEK, Josef MASEK, Josef SLAVIK, Ondrej KOVAC, Jiri NECA, Pavel KULICH, Dominik HREBÍK, Jana STRASKA, Katerina PENCIKOVA, Jirina PROCHAZKOVA, Pavel DIVIS, Stuart MACAULAY, Robert MIKULÍK, Milan RASKA, Miroslav MACHALA and Jaroslav TURÁNEK. Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, vol.~10, No~1, p.~1-13. ISSN~2045-2322. doi:10.1038/s41598-020-60284-z. 2020.
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