Gadolinium labelled nanoliposomes as the platform for MRI
theranostics: in vitro safety study in liver cells and
macrophages

J 2020

Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages

SIMECKOVA, Pavlina, Frantisek HUBATKA, Jan KOTOUCEK, Pavlina KNOTIGOVA TURANEK, Josef MASEK et. al.

Basic information

Original name

Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages

Authors

SIMECKOVA, Pavlina (203 Czech Republic), Frantisek HUBATKA (203 Czech Republic), Jan KOTOUCEK (203 Czech Republic), Pavlina KNOTIGOVA TURANEK (203 Czech Republic), Josef MASEK (203 Czech Republic), Josef SLAVIK (203 Czech Republic), Ondrej KOVAC (203 Czech Republic), Jiri NECA (203 Czech Republic), Pavel KULICH (203 Czech Republic), Dominik HREBÍK (703 Slovakia, belonging to the institution), Jana STRASKA (203 Czech Republic), Katerina PENCIKOVA (203 Czech Republic), Jirina PROCHAZKOVA (203 Czech Republic), Pavel DIVIS (203 Czech Republic), Stuart MACAULAY (826 United Kingdom of Great Britain and Northern Ireland), Robert MIKULÍK (203 Czech Republic, belonging to the institution), Milan RASKA (203 Czech Republic), Miroslav MACHALA (203 Czech Republic) and Jaroslav TURÁNEK (203 Czech Republic, guarantor)

Edition

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2020, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30224 Radiology, nuclear medicine and medical imaging

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.379

RIV identification code

RIV/00216224:14110/20:00116364

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-020-60284-z

UT WoS

000562124000002

Keywords in English

HEPARG CELLS; MOLECULAR-MECHANISMS; OXIDATIVE STRESS; GENE-EXPRESSION; CONTRAST AGENTS; DTPA LIPOSOMES; TOXICITY; GRAPHENE; DISEASES; ENZYMES

Abstract

V originále

Gadolinium (Gd)-based contrast agents are extensively used for magnetic resonance imaging (MRI). Liposomes are potential nanocarrier-based biocompatible platforms for development of new generations of MRI diagnostics. Liposomes with Gd-complexes (Gd-lip) co-encapsulated with thrombolytic agents can serve both for imaging and treatment of various pathological states including stroke. In this study, we evaluated nanosafety of Gd-lip containing PE-DTPA chelating Gd+3 prepared by lipid film hydration method. We detected no cytotoxicity of Gd-lip in human liver cells including cancer HepG2, progenitor (non-differentiated) HepaRG, and differentiated HepaRG cells. Furthermore, no potential side effects of Gd-lip were found using a complex system including general biomarkers of toxicity, such as induction of early response genes, oxidative, heat shock and endoplasmic reticulum stress, DNA damage responses, induction of xenobiotic metabolizing enzymes, and changes in sphingolipid metabolism in differentiated HepaRG. Moreover, Gd-lip did not show pro-inflammatory effects, as assessed in an assay based on activation of inflammasome NLRP3 in a model of human macrophages, and release of eicosanoids from HepaRG cells. In conclusion, this in vitro study indicates potential in vivo safety of Gd-lip with respect to hepatotoxicity and immunopathology caused by inflammation.

Citovat

SIMECKOVA, Pavlina, Frantisek HUBATKA, Jan KOTOUCEK, Pavlina KNOTIGOVA TURANEK, Josef MASEK, Josef SLAVIK, Ondrej KOVAC, Jiri NECA, Pavel KULICH, Dominik HREBÍK, Jana STRASKA, Katerina PENCIKOVA, Jirina PROCHAZKOVA, Pavel DIVIS, Stuart MACAULAY, Robert MIKULÍK, Milan RASKA, Miroslav MACHALA and Jaroslav TURÁNEK. Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2020, vol. 10, No 1, p. 1-13. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-60284-z.

@article{1677829,
   author = {Simeckova, Pavlina and Hubatka, Frantisek and Kotoucek, Jan and Knotigova Turanek, Pavlina and Masek, Josef and Slavik, Josef and Kovac, Ondrej and Neca, Jiri and Kulich, Pavel and Hrebík, Dominik and Straska, Jana and Pencikova, Katerina and Prochazkova, Jirina and Divis, Pavel and Macaulay, Stuart and Mikulík, Robert and Raska, Milan and Machala, Miroslav and Turánek, Jaroslav},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-020-60284-z},
   keywords = {HEPARG CELLS; MOLECULAR-MECHANISMS; OXIDATIVE STRESS; GENE-EXPRESSION; CONTRAST AGENTS; DTPA LIPOSOMES; TOXICITY; GRAPHENE; DISEASES; ENZYMES},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages},
   url = {https://www.nature.com/articles/s41598-020-60284-z.pdf},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1677829
AU  - Simeckova, Pavlina - Hubatka, Frantisek - Kotoucek, Jan - Knotigova Turanek, Pavlina - Masek, Josef - Slavik, Josef - Kovac, Ondrej - Neca, Jiri - Kulich, Pavel - Hrebík, Dominik - Straska, Jana - Pencikova, Katerina - Prochazkova, Jirina - Divis, Pavel - Macaulay, Stuart - Mikulík, Robert - Raska, Milan - Machala, Miroslav - Turánek, Jaroslav
PY  - 2020
TI  - Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages
JF  - Scientific reports
VL  - 10
IS  - 1
SP  - 1-13
EP  - 1-13
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - HEPARG CELLS
KW  - MOLECULAR-MECHANISMS
KW  - OXIDATIVE STRESS
KW  - GENE-EXPRESSION
KW  - CONTRAST AGENTS
KW  - DTPA LIPOSOMES
KW  - TOXICITY
KW  - GRAPHENE
KW  - DISEASES
KW  - ENZYMES
UR  - https://www.nature.com/articles/s41598-020-60284-z.pdf
L2  - https://www.nature.com/articles/s41598-020-60284-z.pdf
N2  - Gadolinium (Gd)-based contrast agents are extensively used for magnetic resonance imaging (MRI). Liposomes are potential nanocarrier-based biocompatible platforms for development of new generations of MRI diagnostics. Liposomes with Gd-complexes (Gd-lip) co-encapsulated with thrombolytic agents can serve both for imaging and treatment of various pathological states including stroke. In this study, we evaluated nanosafety of Gd-lip containing PE-DTPA chelating Gd+3 prepared by lipid film hydration method. We detected no cytotoxicity of Gd-lip in human liver cells including cancer HepG2, progenitor (non-differentiated) HepaRG, and differentiated HepaRG cells. Furthermore, no potential side effects of Gd-lip were found using a complex system including general biomarkers of toxicity, such as induction of early response genes, oxidative, heat shock and endoplasmic reticulum stress, DNA damage responses, induction of xenobiotic metabolizing enzymes, and changes in sphingolipid metabolism in differentiated HepaRG. Moreover, Gd-lip did not show pro-inflammatory effects, as assessed in an assay based on activation of inflammasome NLRP3 in a model of human macrophages, and release of eicosanoids from HepaRG cells. In conclusion, this in vitro study indicates potential in vivo safety of Gd-lip with respect to hepatotoxicity and immunopathology caused by inflammation.
ER  -

SIMECKOVA, Pavlina, Frantisek HUBATKA, Jan KOTOUCEK, Pavlina KNOTIGOVA TURANEK, Josef MASEK, Josef SLAVIK, Ondrej KOVAC, Jiri NECA, Pavel KULICH, Dominik HREBÍK, Jana STRASKA, Katerina PENCIKOVA, Jirina PROCHAZKOVA, Pavel DIVIS, Stuart MACAULAY, Robert MIKULÍK, Milan RASKA, Miroslav MACHALA and Jaroslav TURÁNEK. Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2020, vol.~10, No~1, p.~1-13. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-60284-z.

Detailed Information on Publication Record