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@article{1677829, author = {Simeckova, Pavlina and Hubatka, Frantisek and Kotoucek, Jan and Knotigova Turanek, Pavlina and Masek, Josef and Slavik, Josef and Kovac, Ondrej and Neca, Jiri and Kulich, Pavel and Hrebík, Dominik and Straska, Jana and Pencikova, Katerina and Prochazkova, Jirina and Divis, Pavel and Macaulay, Stuart and Mikulík, Robert and Raska, Milan and Machala, Miroslav and Turánek, Jaroslav}, article_location = {LONDON}, article_number = {1}, doi = {http://dx.doi.org/10.1038/s41598-020-60284-z}, keywords = {HEPARG CELLS; MOLECULAR-MECHANISMS; OXIDATIVE STRESS; GENE-EXPRESSION; CONTRAST AGENTS; DTPA LIPOSOMES; TOXICITY; GRAPHENE; DISEASES; ENZYMES}, language = {eng}, issn = {2045-2322}, journal = {Scientific reports}, title = {Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages}, url = {https://www.nature.com/articles/s41598-020-60284-z.pdf}, volume = {10}, year = {2020} }
TY - JOUR ID - 1677829 AU - Simeckova, Pavlina - Hubatka, Frantisek - Kotoucek, Jan - Knotigova Turanek, Pavlina - Masek, Josef - Slavik, Josef - Kovac, Ondrej - Neca, Jiri - Kulich, Pavel - Hrebík, Dominik - Straska, Jana - Pencikova, Katerina - Prochazkova, Jirina - Divis, Pavel - Macaulay, Stuart - Mikulík, Robert - Raska, Milan - Machala, Miroslav - Turánek, Jaroslav PY - 2020 TI - Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages JF - Scientific reports VL - 10 IS - 1 SP - 1-13 EP - 1-13 PB - NATURE PUBLISHING GROUP SN - 20452322 KW - HEPARG CELLS KW - MOLECULAR-MECHANISMS KW - OXIDATIVE STRESS KW - GENE-EXPRESSION KW - CONTRAST AGENTS KW - DTPA LIPOSOMES KW - TOXICITY KW - GRAPHENE KW - DISEASES KW - ENZYMES UR - https://www.nature.com/articles/s41598-020-60284-z.pdf L2 - https://www.nature.com/articles/s41598-020-60284-z.pdf N2 - Gadolinium (Gd)-based contrast agents are extensively used for magnetic resonance imaging (MRI). Liposomes are potential nanocarrier-based biocompatible platforms for development of new generations of MRI diagnostics. Liposomes with Gd-complexes (Gd-lip) co-encapsulated with thrombolytic agents can serve both for imaging and treatment of various pathological states including stroke. In this study, we evaluated nanosafety of Gd-lip containing PE-DTPA chelating Gd+3 prepared by lipid film hydration method. We detected no cytotoxicity of Gd-lip in human liver cells including cancer HepG2, progenitor (non-differentiated) HepaRG, and differentiated HepaRG cells. Furthermore, no potential side effects of Gd-lip were found using a complex system including general biomarkers of toxicity, such as induction of early response genes, oxidative, heat shock and endoplasmic reticulum stress, DNA damage responses, induction of xenobiotic metabolizing enzymes, and changes in sphingolipid metabolism in differentiated HepaRG. Moreover, Gd-lip did not show pro-inflammatory effects, as assessed in an assay based on activation of inflammasome NLRP3 in a model of human macrophages, and release of eicosanoids from HepaRG cells. In conclusion, this in vitro study indicates potential in vivo safety of Gd-lip with respect to hepatotoxicity and immunopathology caused by inflammation. ER -
SIMECKOVA, Pavlina, Frantisek HUBATKA, Jan KOTOUCEK, Pavlina KNOTIGOVA TURANEK, Josef MASEK, Josef SLAVIK, Ondrej KOVAC, Jiri NECA, Pavel KULICH, Dominik HREBÍK, Jana STRASKA, Katerina PENCIKOVA, Jirina PROCHAZKOVA, Pavel DIVIS, Stuart MACAULAY, Robert MIKULÍK, Milan RASKA, Miroslav MACHALA and Jaroslav TURÁNEK. Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2020, vol.~10, No~1, p.~1-13. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-60284-z.
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