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@article{1679128,
author = {Vrbová, Eliška and Mikalová, Lenka and Grillová, Linda and Pospíšilová, Petra and Strnadel, Radim and Dastychová, Eliška and Kojanova, Martina and Kreidlova, Miluse and Vanousova, Daniela and Rob, Filip and Prochazka, Premysl and Krchňáková, Alena and Vašků, Vladimír and Woznicová, Vladana and Dvořáková Heroldová, Monika and Kuklova, Ivana and Zakoucka, Hana and Šmajs, David},
article_location = {San Francisco},
article_number = {8},
doi = {http://dx.doi.org/10.1371/journal.pone.0237949},
keywords = {syphilis treponeme; PCR detection},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results},
url = {https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable},
volume = {15},
year = {2020}
}
TY - JOUR
ID - 1679128
AU - Vrbová, Eliška - Mikalová, Lenka - Grillová, Linda - Pospíšilová, Petra - Strnadel, Radim - Dastychová, Eliška - Kojanova, Martina - Kreidlova, Miluse - Vanousova, Daniela - Rob, Filip - Prochazka, Premysl - Krchňáková, Alena - Vašků, Vladimír - Woznicová, Vladana - Dvořáková Heroldová, Monika - Kuklova, Ivana - Zakoucka, Hana - Šmajs, David
PY - 2020
TI - A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results
JF - Plos one
VL - 15
IS - 8
SP - 1-11
EP - 1-11
PB - Public Library of Science
SN - 19326203
KW - syphilis treponeme
KW - PCR detection
UR - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable
L2 - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable
N2 - Syphilis, caused byTreponema pallidumssp.pallidum(TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.
ER -
VRBOVÁ, Eliška, Lenka MIKALOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Radim STRNADEL, Eliška DASTYCHOVÁ, Martina KOJANOVA, Miluse KREIDLOVA, Daniela VANOUSOVA, Filip ROB, Premysl PROCHAZKA, Alena KRCHŇÁKOVÁ, Vladimír VAŠKŮ, Vladana WOZNICOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Ivana KUKLOVA, Hana ZAKOUCKA and David ŠMAJS. A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results. \textit{Plos one}. San Francisco: Public Library of Science, 2020, vol.~15, No~8, p.~1-11. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0237949.
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