Bioreactor Processed Stromal Cell Seeding and Cultivation on
Decellularized Pericardium Patches for Cardiovascular Use

J 2020

Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use

MATEJKA, Roman, Miroslav KONARIK, Jana STEPANOVSKA, Jan LIPENSKY, Jaroslav CHLUPAC et. al.

Basic information

Original name

Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use

Authors

MATEJKA, Roman (203 Czech Republic, guarantor), Miroslav KONARIK (203 Czech Republic), Jana STEPANOVSKA (203 Czech Republic), Jan LIPENSKY (203 Czech Republic), Jaroslav CHLUPAC (203 Czech Republic), Daniel TUREK (203 Czech Republic), Simon PRAZAK (203 Czech Republic), Antonin BROZ (203 Czech Republic), Zuzana SIMUNKOVA (203 Czech Republic), Iveta MRAZOVA (203 Czech Republic), Serhij FOROSTYAK (203 Czech Republic, belonging to the institution), Peter KNEPPO (203 Czech Republic), Jozef ROSINA (203 Czech Republic), Lucie BACAKOVA (203 Czech Republic) and Jan PIRK (203 Czech Republic)

Edition

Applied Sciences-Basel, BASEL, MDPI AG, 2020, 2076-3417

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30201 Cardiac and Cardiovascular systems

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.679

RIV identification code

RIV/00216224:14110/20:00116429

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/app10165473

UT WoS

000564790600001

Keywords in English

bioreactor; cardiovascular patch; decellularization; recellularization; pericardium

Abstract

V originále

Featured Application In this study, we have prepared decellularized pericardium repopulated with adipose tissue-derived stromal cells for potential use as implantable cardiovascular patches. Novel optimized dynamic decellularization and recellularization systems have been used and demonstrated. In this bioreactor, the stromal cells deeply repopulated the full thickness of the matrix, and they pre-differentiated towards the smooth muscle cell phenotype by applying cyclic pressure stimulation. Thus, these dynamically recellularized patches resemble vascular tunica media. These grafts may be further applied in animal experiments to assess surface endothelialization and in vivo remodelling. Animal tissue is of large potential availability and decellularization renders the matrix non-immunogenic. Autologous adipose cells for recellularization can be harvested through small biopsy in human patients with cardiovascular disease. A potential application of this approach is manufacturing a tissue-engineered cardiovascular patch with improved biocompatibility for the surgical repair of the human heart or vessels, such as the carotid artery or femoral artery. (1)Background: Decellularized xenogeneic tissues are promising matrices for developing tissue-engineered cardiovascular grafts. In vitro recellularization of these tissues with stromal cells can provide a better in vivo remodelling and a lower thrombogenicity of the graft. The process of recellularization can be accelerated using a cultivation bioreactor simulating physiological conditions and stimuli. (2)Methods: Porcine pericardium was decellularized using a custom-built decellularization system with an optimized protocol. Autologous porcine adipose-derived stromal cells (PrASCs), isolated from the subcutaneous fat tissue, were used for recellularizing the decellularized pericardium. A custom cultivation bioreactor allowing the fixing of the decellularized tissue into a special cultivation chamber was created. The bioreactor maintained micro-perfusion and pulsatile pressure stimulation in order to promote the ingrowth of PrASCs inside the tissue and their differentiation. (3)Results: The dynamic cultivation promoted the ingrowth of cells into the decellularized tissue. Under static conditions, the cells penetrated only to the depth of 50 mu m, whereas under dynamic conditions, the tissue was colonized up to 250 mu m. The dynamic cultivation also supported the cell differentiation towards smooth muscle cells (SMCs). In order to ensure homogeneous cell colonization of the decellularized matrices, the bioreactor was designed to allow seeding of the cells from both sides of the tissue prior to the stimulation. In this case, the decellularized tissue was recolonized with cells within 5 days of dynamic cultivation. (4)Conclusions: Our newly designed dynamic bioreactor markedly accelerated the colonization of decellularized pericardium with ASCs and cell differentiation towards the SMC phenotype.

Citovat

MATEJKA, Roman, Miroslav KONARIK, Jana STEPANOVSKA, Jan LIPENSKY, Jaroslav CHLUPAC, Daniel TUREK, Simon PRAZAK, Antonin BROZ, Zuzana SIMUNKOVA, Iveta MRAZOVA, Serhij FOROSTYAK, Peter KNEPPO, Jozef ROSINA, Lucie BACAKOVA and Jan PIRK. Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use. Applied Sciences-Basel. BASEL: MDPI AG, 2020, vol. 10, No 16, p. 1-22. ISSN 2076-3417. Available from: https://dx.doi.org/10.3390/app10165473.

@article{1679130,
   author = {Matejka, Roman and Konarik, Miroslav and Stepanovska, Jana and Lipensky, Jan and Chlupac, Jaroslav and Turek, Daniel and Prazak, Simon and Broz, Antonin and Simunkova, Zuzana and Mrazova, Iveta and Forostyak, Serhij and Kneppo, Peter and Rosina, Jozef and Bacakova, Lucie and Pirk, Jan},
   article_location = {BASEL},
   article_number = {16},
   doi = {http://dx.doi.org/10.3390/app10165473},
   keywords = {bioreactor; cardiovascular patch; decellularization; recellularization; pericardium},
   language = {eng},
   issn = {2076-3417},
   journal = {Applied Sciences-Basel},
   title = {Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use},
   url = {https://www.mdpi.com/2076-3417/10/16/5473},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1679130
AU  - Matejka, Roman - Konarik, Miroslav - Stepanovska, Jana - Lipensky, Jan - Chlupac, Jaroslav - Turek, Daniel - Prazak, Simon - Broz, Antonin - Simunkova, Zuzana - Mrazova, Iveta - Forostyak, Serhij - Kneppo, Peter - Rosina, Jozef - Bacakova, Lucie - Pirk, Jan
PY  - 2020
TI  - Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use
JF  - Applied Sciences-Basel
VL  - 10
IS  - 16
SP  - 1-22
EP  - 1-22
PB  - MDPI AG
SN  - 20763417
KW  - bioreactor
KW  - cardiovascular patch
KW  - decellularization
KW  - recellularization
KW  - pericardium
UR  - https://www.mdpi.com/2076-3417/10/16/5473
L2  - https://www.mdpi.com/2076-3417/10/16/5473
N2  - Featured Application In this study, we have prepared decellularized pericardium repopulated with adipose tissue-derived stromal cells for potential use as implantable cardiovascular patches. Novel optimized dynamic decellularization and recellularization systems have been used and demonstrated. In this bioreactor, the stromal cells deeply repopulated the full thickness of the matrix, and they pre-differentiated towards the smooth muscle cell phenotype by applying cyclic pressure stimulation. Thus, these dynamically recellularized patches resemble vascular tunica media. These grafts may be further applied in animal experiments to assess surface endothelialization and in vivo remodelling. Animal tissue is of large potential availability and decellularization renders the matrix non-immunogenic. Autologous adipose cells for recellularization can be harvested through small biopsy in human patients with cardiovascular disease. A potential application of this approach is manufacturing a tissue-engineered cardiovascular patch with improved biocompatibility for the surgical repair of the human heart or vessels, such as the carotid artery or femoral artery. (1)Background: Decellularized xenogeneic tissues are promising matrices for developing tissue-engineered cardiovascular grafts. In vitro recellularization of these tissues with stromal cells can provide a better in vivo remodelling and a lower thrombogenicity of the graft. The process of recellularization can be accelerated using a cultivation bioreactor simulating physiological conditions and stimuli. (2)Methods: Porcine pericardium was decellularized using a custom-built decellularization system with an optimized protocol. Autologous porcine adipose-derived stromal cells (PrASCs), isolated from the subcutaneous fat tissue, were used for recellularizing the decellularized pericardium. A custom cultivation bioreactor allowing the fixing of the decellularized tissue into a special cultivation chamber was created. The bioreactor maintained micro-perfusion and pulsatile pressure stimulation in order to promote the ingrowth of PrASCs inside the tissue and their differentiation. (3)Results: The dynamic cultivation promoted the ingrowth of cells into the decellularized tissue. Under static conditions, the cells penetrated only to the depth of 50 mu m, whereas under dynamic conditions, the tissue was colonized up to 250 mu m. The dynamic cultivation also supported the cell differentiation towards smooth muscle cells (SMCs). In order to ensure homogeneous cell colonization of the decellularized matrices, the bioreactor was designed to allow seeding of the cells from both sides of the tissue prior to the stimulation. In this case, the decellularized tissue was recolonized with cells within 5 days of dynamic cultivation. (4)Conclusions: Our newly designed dynamic bioreactor markedly accelerated the colonization of decellularized pericardium with ASCs and cell differentiation towards the SMC phenotype.
ER  -

MATEJKA, Roman, Miroslav KONARIK, Jana STEPANOVSKA, Jan LIPENSKY, Jaroslav CHLUPAC, Daniel TUREK, Simon PRAZAK, Antonin BROZ, Zuzana SIMUNKOVA, Iveta MRAZOVA, Serhij FOROSTYAK, Peter KNEPPO, Jozef ROSINA, Lucie BACAKOVA and Jan PIRK. Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use. \textit{Applied Sciences-Basel}. BASEL: MDPI AG, 2020, vol.~10, No~16, p.~1-22. ISSN~2076-3417. Available from: https://dx.doi.org/10.3390/app10165473.

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