J 2020

Characterization and in vitro Analysis of Probiotic-Derived Peptides Against Multi Drug Resistance Bacterial Infections

MAZUMDAR, Aninda, Yazan HADDAD, Vishma Pratap SUR, Vedran MILOSAVLJEVIC, Sukanya BHOWMICK et. al.

Basic information

Original name

Characterization and in vitro Analysis of Probiotic-Derived Peptides Against Multi Drug Resistance Bacterial Infections

Authors

MAZUMDAR, Aninda (guarantor), Yazan HADDAD, Vishma Pratap SUR, Vedran MILOSAVLJEVIC, Sukanya BHOWMICK, Hana MICHÁLKOVÁ (203 Czech Republic), Roman GURÁŇ (203 Czech Republic), Radek VESELÝ (203 Czech Republic, belonging to the institution) and Amitava MOULICK

Edition

Frontiers in Microbiology, Lausanne, Frontiers Media SA, 2020, 1664-302X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.640

RIV identification code

RIV/00216224:14110/20:00116440

Organization unit

Faculty of Medicine

UT WoS

000570552300001

Keywords in English

antibacterial peptides antibiotics multidrug resistance bacteria infections

Tags

Tags

International impact, Reviewed
Změněno: 2/10/2020 09:02, Mgr. Tereza Miškechová

Abstract

V originále

An inexorable switch from antibiotics has become a major desideratum to overcome antibiotic resistance. Bacteriocin fromLactobacillus casei, a cardinal probiotic was used to design novel antibacterial peptides named as Probiotic Bacteriocin Derived and Modified (PBDM) peptides (PBDM1: YKWFAHLIKGLC and PBDM2: YKWFRHLIKKLC). The loop-shaped 3D structure of peptides was characterizedin silicovia molecular dynamics simulation as well as biophysically via spectroscopic methods. Thereafter,in vitroresults against multidrug resistant bacterial strains and hospital samples demonstrated the strong antimicrobial activity of PBDM peptides. Further,in vivostudies with PBDM peptides showed downright recovery of balb/c mice from Vancomycin ResistantStaphylococcus aureus(VRSA) infection to its healthy condition. Thereafter,in vitrostudy with human epithelial cells showed no significant cytotoxic effects with high biocompatibility and good hemocompatibility. In conclusion, PBDM peptides displayed significant antibacterial activity against certain drug resistant bacteria which cause infections in human beings. Future analysis are required to unveil its mechanism of action in order to execute it as an alternative to antibiotics.

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR