Adipophilin and perilipin 3 positively correlate with total
lipid content in human breast milk

J 2020

Adipophilin and perilipin 3 positively correlate with total lipid content in human breast milk

PAVLOVÁ, Tereza, Zdeněk SPÁČIL, Veronika VIDOVÁ, Filip ZLÁMAL, Eliška ČECHOVÁ et. al.

Basic information

Original name

Adipophilin and perilipin 3 positively correlate with total lipid content in human breast milk

Authors

PAVLOVÁ, Tereza (203 Czech Republic, belonging to the institution), Zdeněk SPÁČIL (203 Czech Republic, belonging to the institution), Veronika VIDOVÁ (203 Czech Republic, belonging to the institution), Filip ZLÁMAL (203 Czech Republic, belonging to the institution), Eliška ČECHOVÁ (203 Czech Republic, belonging to the institution), Zuzana HODICKÁ (203 Czech Republic) and Julie DOBROVOLNÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific reports, London, Nature Publishing Group, 2020, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10700 1.7 Other natural sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.379

RIV identification code

RIV/00216224:14310/20:00114318

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-019-57241-w

UT WoS

000551443900011

Keywords in English

Lipids; Membrane proteins; Peptides

Abstract

V originále

Lipids are secreted into milk as bilayer-coated structures: milk fat globules (MFGs). Adipophilin (ADRP) and perilipin 3 (TIP47) are associated with MFGs in human breast milk; however, the role of these proteins in milk lipid secretion is not fully understood. The study aimed to investigate levels of ADRP, TIP47 and total lipid content in human breast milk, their mutual correlations, and dynamics during lactation. Milk samples from 22 healthy lactating women (Caucasian, Central European) were collected at five time points during lactation (1-3, 12-14, 29-30, 88-90 and 178-180 days postpartum). Mass spectrometry-based method was used for quantification of ADRP and TIP47 in the samples. The gravimetric method was used to determine milk total lipid content. We observed distinctive trends in ADRP, TIP47 levels and lipid content in human breast milk during the first six months of lactation. We also found a significant association between lipid content and ADRP, lipid content and TIP47, and ADRP and TIP47 concentrations in breast milk at all sampling points. A mass spectrometry-based method was developed for quantifying ADRP and TIP47 in human breast milk. Strong mutual correlations were found between ADRP, TIP47 and total lipid content in human breast milk.

Links

EF15_003/0000469, research and development project

Name: Cetocoen Plus

GJ17-24592Y, research and development project

Name: Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PAVLOVÁ, Tereza, Zdeněk SPÁČIL, Veronika VIDOVÁ, Filip ZLÁMAL, Eliška ČECHOVÁ, Zuzana HODICKÁ and Julie DOBROVOLNÁ. Adipophilin and perilipin 3 positively correlate with total lipid content in human breast milk. Scientific reports. London: Nature Publishing Group, 2020, vol. 10, No 1, p. 1-9. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-57241-w.

@article{1679604,
   author = {Pavlová, Tereza and Spáčil, Zdeněk and Vidová, Veronika and Zlámal, Filip and Čechová, Eliška and Hodická, Zuzana and Dobrovolná, Julie},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-019-57241-w},
   keywords = {Lipids; Membrane proteins; Peptides},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Adipophilin and perilipin 3 positively correlate with total lipid content in human breast milk},
   url = {https://www.nature.com/articles/s41598-019-57241-w},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1679604
AU  - Pavlová, Tereza - Spáčil, Zdeněk - Vidová, Veronika - Zlámal, Filip - Čechová, Eliška - Hodická, Zuzana - Dobrovolná, Julie
PY  - 2020
TI  - Adipophilin and perilipin 3 positively correlate with total lipid content in human breast milk
JF  - Scientific reports
VL  - 10
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - Nature Publishing Group
SN  - 20452322
KW  - Lipids
KW  - Membrane proteins
KW  - Peptides
UR  - https://www.nature.com/articles/s41598-019-57241-w
L2  - https://www.nature.com/articles/s41598-019-57241-w
N2  - Lipids are secreted into milk as bilayer-coated structures: milk fat globules (MFGs). Adipophilin (ADRP) and perilipin 3 (TIP47) are associated with MFGs in human breast milk; however, the role of these proteins in milk lipid secretion is not fully understood. The study aimed to investigate levels of ADRP, TIP47 and total lipid content in human breast milk, their mutual correlations, and dynamics during lactation. Milk samples from 22 healthy lactating women (Caucasian, Central European) were collected at five time points during lactation (1-3, 12-14, 29-30, 88-90 and 178-180 days postpartum). Mass spectrometry-based method was used for quantification of ADRP and TIP47 in the samples. The gravimetric method was used to determine milk total lipid content. We observed distinctive trends in ADRP, TIP47 levels and lipid content in human breast milk during the first six months of lactation. We also found a significant association between lipid content and ADRP, lipid content and TIP47, and ADRP and TIP47 concentrations in breast milk at all sampling points. A mass spectrometry-based method was developed for quantifying ADRP and TIP47 in human breast milk. Strong mutual correlations were found between ADRP, TIP47 and total lipid content in human breast milk.
ER  -

PAVLOVÁ, Tereza, Zdeněk SPÁČIL, Veronika VIDOVÁ, Filip ZLÁMAL, Eliška ČECHOVÁ, Zuzana HODICKÁ and Julie DOBROVOLNÁ. Adipophilin and perilipin 3 positively correlate with total lipid content in human breast milk. \textit{Scientific reports}. London: Nature Publishing Group, 2020, vol.~10, No~1, p.~1-9. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-57241-w.

Detailed Information on Publication Record