Involvement of the cbb(3)-Type Terminal Oxidase in Growth
Competition of Bacteria, Biofilm Formation, and in Switching
between Denitrification and Aerobic Respiration

J 2020

Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration

KUČERA, Igor and Vojtěch SEDLÁČEK

Basic information

Original name

Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration

Name in Czech

Účast terminální oxidasy typu cbb(3) při růstové kompetici bakterií, tvorbě biofilmu a přepnutí mezi denitrifikací a aerobní respirací

Authors

KUČERA, Igor (203 Czech Republic, guarantor, belonging to the institution) and Vojtěch SEDLÁČEK (203 Czech Republic, belonging to the institution)

Edition

Microorganisms, Basel, MDPI, 2020, 2076-2607

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.128

RIV identification code

RIV/00216224:14310/20:00114320

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/microorganisms8081230

UT WoS

000564643700001

Keywords (in Czech)

respirační řetězec; terminální oxidasy; denitrifikace; rozvětvený tok elektronů; biofilm

Keywords in English

respiratory chain; terminal oxidases; denitrification; branched electron flow; biofilm

Abstract

V originále

Paracoccus denitrificans has a branched electron transport chain with three terminal oxidases transferring electrons to molecular oxygen, namely aa(3)-type and cbb(3)-type cytochrome c oxidases and ba(3)-type ubiquinol oxidase. In the present study, we focused on strains expressing only one of these enzymes. The competition experiments showed that possession of cbb(3)-type oxidase confers significant fitness advantage during oxygen-limited growth and supports the biofilm lifestyle. The aa(3)-type oxidase was shown to allow rapid aerobic growth at a high oxygen supply. Activity of the denitrification pathway that had been expressed in cells grown anaerobically with nitrate was fully inhibitable by oxygen only in wild-type and cbb(3) strains, while in strains aa(3) and ba(3) dinitrogen production from nitrate and oxygen consumption occurred simultaneously. Together, the results highlight the importance of the cbb(3)-type oxidase for the denitrification phenotype and suggest a way of obtaining novel bacterial strains capable of aerobic denitrification.

Links

GA16-18476S, research and development project

Name: Oxidační stres u denitrifikačních baktérií: objasnění funkce zúčastněných proteinů a možných dopadů na životní prostředí

Investor: Czech Science Foundation

Citovat

KUČERA, Igor and Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. Microorganisms. Basel: MDPI, 2020, vol. 8, No 8, p. 1-11. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms8081230.

@article{1679979,
   author = {Kučera, Igor and Sedláček, Vojtěch},
   article_location = {Basel},
   article_number = {8},
   doi = {http://dx.doi.org/10.3390/microorganisms8081230},
   keywords = {respiratory chain; terminal oxidases; denitrification; branched electron flow; biofilm},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration},
   url = {https://doi.org/10.3390/microorganisms8081230},
   volume = {8},
   year = {2020}
}

TY  - JOUR
ID  - 1679979
AU  - Kučera, Igor - Sedláček, Vojtěch
PY  - 2020
TI  - Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration
JF  - Microorganisms
VL  - 8
IS  - 8
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 20762607
KW  - respiratory chain
KW  - terminal oxidases
KW  - denitrification
KW  - branched electron flow
KW  - biofilm
UR  - https://doi.org/10.3390/microorganisms8081230
L2  - https://doi.org/10.3390/microorganisms8081230
N2  - Paracoccus denitrificans has a branched electron transport chain with three terminal oxidases transferring electrons to molecular oxygen, namely aa(3)-type and cbb(3)-type cytochrome c oxidases and ba(3)-type ubiquinol oxidase. In the present study, we focused on strains expressing only one of these enzymes. The competition experiments showed that possession of cbb(3)-type oxidase confers significant fitness advantage during oxygen-limited growth and supports the biofilm lifestyle. The aa(3)-type oxidase was shown to allow rapid aerobic growth at a high oxygen supply. Activity of the denitrification pathway that had been expressed in cells grown anaerobically with nitrate was fully inhibitable by oxygen only in wild-type and cbb(3) strains, while in strains aa(3) and ba(3) dinitrogen production from nitrate and oxygen consumption occurred simultaneously. Together, the results highlight the importance of the cbb(3)-type oxidase for the denitrification phenotype and suggest a way of obtaining novel bacterial strains capable of aerobic denitrification.
ER  -

KUČERA, Igor and Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. \textit{Microorganisms}. Basel: MDPI, 2020, vol.~8, No~8, p.~1-11. ISSN~2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms8081230.

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