KUČERA, Igor and Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. Microorganisms. Basel: MDPI, 2020, vol. 8, No 8, p. 1-11. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms8081230. |
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@article{1679979, author = {Kučera, Igor and Sedláček, Vojtěch}, article_location = {Basel}, article_number = {8}, doi = {http://dx.doi.org/10.3390/microorganisms8081230}, keywords = {respiratory chain; terminal oxidases; denitrification; branched electron flow; biofilm}, language = {eng}, issn = {2076-2607}, journal = {Microorganisms}, title = {Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration}, url = {https://doi.org/10.3390/microorganisms8081230}, volume = {8}, year = {2020} }
TY - JOUR ID - 1679979 AU - Kučera, Igor - Sedláček, Vojtěch PY - 2020 TI - Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration JF - Microorganisms VL - 8 IS - 8 SP - 1-11 EP - 1-11 PB - MDPI SN - 20762607 KW - respiratory chain KW - terminal oxidases KW - denitrification KW - branched electron flow KW - biofilm UR - https://doi.org/10.3390/microorganisms8081230 L2 - https://doi.org/10.3390/microorganisms8081230 N2 - Paracoccus denitrificans has a branched electron transport chain with three terminal oxidases transferring electrons to molecular oxygen, namely aa(3)-type and cbb(3)-type cytochrome c oxidases and ba(3)-type ubiquinol oxidase. In the present study, we focused on strains expressing only one of these enzymes. The competition experiments showed that possession of cbb(3)-type oxidase confers significant fitness advantage during oxygen-limited growth and supports the biofilm lifestyle. The aa(3)-type oxidase was shown to allow rapid aerobic growth at a high oxygen supply. Activity of the denitrification pathway that had been expressed in cells grown anaerobically with nitrate was fully inhibitable by oxygen only in wild-type and cbb(3) strains, while in strains aa(3) and ba(3) dinitrogen production from nitrate and oxygen consumption occurred simultaneously. Together, the results highlight the importance of the cbb(3)-type oxidase for the denitrification phenotype and suggest a way of obtaining novel bacterial strains capable of aerobic denitrification. ER -
KUČERA, Igor and Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. \textit{Microorganisms}. Basel: MDPI, 2020, vol.~8, No~8, p.~1-11. ISSN~2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms8081230.
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