| KUČERA, Igor and Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. Microorganisms. Basel: MDPI, 2020, vol. 8, No 8, p. 1-11. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms8081230. |
Other formats:
BibTeX
LaTeX
RIS
@article{1679979,
author = {Kučera, Igor and Sedláček, Vojtěch},
article_location = {Basel},
article_number = {8},
doi = {http://dx.doi.org/10.3390/microorganisms8081230},
keywords = {respiratory chain; terminal oxidases; denitrification; branched electron flow; biofilm},
language = {eng},
issn = {2076-2607},
journal = {Microorganisms},
title = {Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration},
url = {https://doi.org/10.3390/microorganisms8081230},
volume = {8},
year = {2020}
}
TY - JOUR
ID - 1679979
AU - Kučera, Igor - Sedláček, Vojtěch
PY - 2020
TI - Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration
JF - Microorganisms
VL - 8
IS - 8
SP - 1-11
EP - 1-11
PB - MDPI
SN - 20762607
KW - respiratory chain
KW - terminal oxidases
KW - denitrification
KW - branched electron flow
KW - biofilm
UR - https://doi.org/10.3390/microorganisms8081230
L2 - https://doi.org/10.3390/microorganisms8081230
N2 - Paracoccus denitrificans has a branched electron transport chain with three terminal oxidases transferring electrons to molecular oxygen, namely aa(3)-type and cbb(3)-type cytochrome c oxidases and ba(3)-type ubiquinol oxidase. In the present study, we focused on strains expressing only one of these enzymes. The competition experiments showed that possession of cbb(3)-type oxidase confers significant fitness advantage during oxygen-limited growth and supports the biofilm lifestyle. The aa(3)-type oxidase was shown to allow rapid aerobic growth at a high oxygen supply. Activity of the denitrification pathway that had been expressed in cells grown anaerobically with nitrate was fully inhibitable by oxygen only in wild-type and cbb(3) strains, while in strains aa(3) and ba(3) dinitrogen production from nitrate and oxygen consumption occurred simultaneously. Together, the results highlight the importance of the cbb(3)-type oxidase for the denitrification phenotype and suggest a way of obtaining novel bacterial strains capable of aerobic denitrification.
ER -
KUČERA, Igor and Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. \textit{Microorganisms}. Basel: MDPI, 2020, vol.~8, No~8, p.~1-11. ISSN~2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms8081230.
|
