Applicability of capillary electrophoresis-frontal analysis for
displacement studies: effect of several drugs on L-tryptophan
and lidocaine binding to human serum albumin

J 2020

Applicability of capillary electrophoresis-frontal analysis for displacement studies: effect of several drugs on L-tryptophan and lidocaine binding to human serum albumin

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ and Zdeněk GLATZ

Basic information

Original name

Applicability of capillary electrophoresis-frontal analysis for displacement studies: effect of several drugs on L-tryptophan and lidocaine binding to human serum albumin

Authors

NEVÍDALOVÁ, Hana (203 Czech Republic, belonging to the institution), Lenka MICHALCOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journa of Separation Science, Weinheim, Wiley-VCH, 2020, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.645

RIV identification code

RIV/00216224:14310/20:00114321

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/jssc.202000594

UT WoS

000582531000001

Keywords (in Czech)

CE; albumin; lečivo

Keywords in English

CE; Albumin; Drug

Abstract

ORIG CZ

V originále

In this study, the binding characteristics of six drugs with human serum albumin, the most abundant protein in human plasma, were determined by capillary electrophoresis-frontal analysis, and the obtained values of binding parameters were compared with the literature data. The affect of several drugs and site markers on the binding of L-tryptophan and lidocaine to human serum albumin was investigated in subsequent displacement studies which thus demonstrated the usability of capillary electrophoresis as an automated high-throughput screening method for drug-protein binding studies.

In Czech

Byly provedeny vytěsňovací studie.

Links

GA19-08358S, research and development project

Name: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Czech Science Foundation

Citovat

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ and Zdeněk GLATZ. Applicability of capillary electrophoresis-frontal analysis for displacement studies: effect of several drugs on L-tryptophan and lidocaine binding to human serum albumin. Journa of Separation Science. Weinheim: Wiley-VCH, 2020, vol. 43, No 22, p. 4225-4233. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.202000594.

@article{1679993,
   author = {Nevídalová, Hana and Michalcová, Lenka and Glatz, Zdeněk},
   article_location = {Weinheim},
   article_number = {22},
   doi = {http://dx.doi.org/10.1002/jssc.202000594},
   keywords = {CE; Albumin; Drug},
   language = {eng},
   issn = {1615-9306},
   journal = {Journa of Separation Science},
   title = {Applicability of capillary electrophoresis-frontal analysis for displacement studies: effect of several drugs on L-tryptophan and lidocaine binding to human serum albumin},
   url = {https://doi.org/10.1002/jssc.202000594},
   volume = {43},
   year = {2020}
}

TY  - JOUR
ID  - 1679993
AU  - Nevídalová, Hana - Michalcová, Lenka - Glatz, Zdeněk
PY  - 2020
TI  - Applicability of capillary electrophoresis-frontal analysis for displacement studies: effect of several drugs on L-tryptophan and lidocaine binding to human serum albumin
JF  - Journa of Separation Science
VL  - 43
IS  - 22
SP  - 4225-4233
EP  - 4225-4233
PB  - Wiley-VCH
SN  - 16159306
KW  - CE
KW  - Albumin
KW  - Drug
UR  - https://doi.org/10.1002/jssc.202000594
L2  - https://doi.org/10.1002/jssc.202000594
N2  - In this study, the binding characteristics of six drugs with human serum albumin, the most abundant protein in human plasma, were determined by capillary electrophoresis-frontal analysis, and the obtained values of binding parameters were compared with the literature data. The affect of several drugs and site markers on the binding of L-tryptophan and lidocaine to human serum albumin was investigated in subsequent displacement studies which thus demonstrated the usability of capillary electrophoresis as an automated high-throughput screening method for drug-protein binding studies.
ER  -

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ and Zdeněk GLATZ. Applicability of capillary electrophoresis-frontal analysis for displacement studies: effect of several drugs on L-tryptophan and lidocaine binding to human serum albumin. \textit{Journa of Separation Science}. Weinheim: Wiley-VCH, 2020, vol.~43, No~22, p.~4225-4233. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.202000594.

Detailed Information on Publication Record