BOHMOVA, Jana, Marek LUBUSKY, Iva HOLUSKOVA, Martina STUDNICKOVA, Romana KRATOCHVILOVA, Eva KREJCIRIKOVA, Veronika DURDOVA, Tereza KRATOCHVILOVA, Ladislav DUŠEK, Martin PROCHAZKA and Radek VODICKA. Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma. Diagnostics. Basel: MDPI, 2020, vol. 10, No 8, p. 1-15. ISSN 2075-4418. Available from: https://dx.doi.org/10.3390/diagnostics10080564. |
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@article{1680420, author = {Bohmova, Jana and Lubusky, Marek and Holuskova, Iva and Studnickova, Martina and Kratochvilova, Romana and Krejcirikova, Eva and Durdova, Veronika and Kratochvilova, Tereza and Dušek, Ladislav and Prochazka, Martin and Vodicka, Radek}, article_location = {Basel}, article_number = {8}, doi = {http://dx.doi.org/10.3390/diagnostics10080564}, keywords = {non-invasive fetal genotyping; RHDgene; cell-free fetal DNA; real-time PCR; QF PCR; Rh blood group system; red blood cell alloimmunization; hemolytic disease of the fetus and newborn}, language = {eng}, issn = {2075-4418}, journal = {Diagnostics}, title = {Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma}, url = {https://www.mdpi.com/2075-4418/10/8/564}, volume = {10}, year = {2020} }
TY - JOUR ID - 1680420 AU - Bohmova, Jana - Lubusky, Marek - Holuskova, Iva - Studnickova, Martina - Kratochvilova, Romana - Krejcirikova, Eva - Durdova, Veronika - Kratochvilova, Tereza - Dušek, Ladislav - Prochazka, Martin - Vodicka, Radek PY - 2020 TI - Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma JF - Diagnostics VL - 10 IS - 8 SP - 1-15 EP - 1-15 PB - MDPI SN - 20754418 KW - non-invasive fetal genotyping KW - RHDgene KW - cell-free fetal DNA KW - real-time PCR KW - QF PCR KW - Rh blood group system KW - red blood cell alloimmunization KW - hemolytic disease of the fetus and newborn UR - https://www.mdpi.com/2075-4418/10/8/564 L2 - https://www.mdpi.com/2075-4418/10/8/564 N2 - Noninvasive fetalRHDgenotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetalRHDgenotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetalRHDgenotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from theRHDgene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of theRHDgenotyping. TheRHDgenotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict theRHDgenotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetalRHDallele from the plasma of RhD-negative pregnant women. ER -
BOHMOVA, Jana, Marek LUBUSKY, Iva HOLUSKOVA, Martina STUDNICKOVA, Romana KRATOCHVILOVA, Eva KREJCIRIKOVA, Veronika DURDOVA, Tereza KRATOCHVILOVA, Ladislav DUŠEK, Martin PROCHAZKA and Radek VODICKA. Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma. \textit{Diagnostics}. Basel: MDPI, 2020, vol.~10, No~8, p.~1-15. ISSN~2075-4418. Available from: https://dx.doi.org/10.3390/diagnostics10080564.
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