Two Reliable Methodical Approaches for Non-Invasive RHD
Genotyping of a Fetus from Maternal Plasma

J 2020

Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma

BOHMOVA, Jana, Marek LUBUSKY, Iva HOLUSKOVA, Martina STUDNICKOVA, Romana KRATOCHVILOVA et. al.

Basic information

Original name

Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma

Authors

BOHMOVA, Jana (203 Czech Republic), Marek LUBUSKY (203 Czech Republic), Iva HOLUSKOVA (203 Czech Republic), Martina STUDNICKOVA (203 Czech Republic), Romana KRATOCHVILOVA (203 Czech Republic), Eva KREJCIRIKOVA (203 Czech Republic), Veronika DURDOVA (203 Czech Republic), Tereza KRATOCHVILOVA (203 Czech Republic), Ladislav DUŠEK (203 Czech Republic, belonging to the institution), Martin PROCHAZKA (203 Czech Republic) and Radek VODICKA (203 Czech Republic, guarantor)

Edition

Diagnostics, Basel, MDPI, 2020, 2075-4418

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30218 General and internal medicine

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.706

RIV identification code

RIV/00216224:14110/20:00116484

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/diagnostics10080564

UT WoS

000568020600001

Keywords in English

non-invasive fetal genotyping; RHDgene; cell-free fetal DNA; real-time PCR; QF PCR; Rh blood group system; red blood cell alloimmunization; hemolytic disease of the fetus and newborn

Abstract

V originále

Noninvasive fetalRHDgenotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetalRHDgenotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetalRHDgenotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from theRHDgene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of theRHDgenotyping. TheRHDgenotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict theRHDgenotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetalRHDallele from the plasma of RhD-negative pregnant women.

Citovat

BOHMOVA, Jana, Marek LUBUSKY, Iva HOLUSKOVA, Martina STUDNICKOVA, Romana KRATOCHVILOVA, Eva KREJCIRIKOVA, Veronika DURDOVA, Tereza KRATOCHVILOVA, Ladislav DUŠEK, Martin PROCHAZKA and Radek VODICKA. Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma. Diagnostics. Basel: MDPI, 2020, vol. 10, No 8, p. 1-15. ISSN 2075-4418. Available from: https://dx.doi.org/10.3390/diagnostics10080564.

@article{1680420,
   author = {Bohmova, Jana and Lubusky, Marek and Holuskova, Iva and Studnickova, Martina and Kratochvilova, Romana and Krejcirikova, Eva and Durdova, Veronika and Kratochvilova, Tereza and Dušek, Ladislav and Prochazka, Martin and Vodicka, Radek},
   article_location = {Basel},
   article_number = {8},
   doi = {http://dx.doi.org/10.3390/diagnostics10080564},
   keywords = {non-invasive fetal genotyping; RHDgene; cell-free fetal DNA; real-time PCR; QF PCR; Rh blood group system; red blood cell alloimmunization; hemolytic disease of the fetus and newborn},
   language = {eng},
   issn = {2075-4418},
   journal = {Diagnostics},
   title = {Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma},
   url = {https://www.mdpi.com/2075-4418/10/8/564},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1680420
AU  - Bohmova, Jana - Lubusky, Marek - Holuskova, Iva - Studnickova, Martina - Kratochvilova, Romana - Krejcirikova, Eva - Durdova, Veronika - Kratochvilova, Tereza - Dušek, Ladislav - Prochazka, Martin - Vodicka, Radek
PY  - 2020
TI  - Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma
JF  - Diagnostics
VL  - 10
IS  - 8
SP  - 1-15
EP  - 1-15
PB  - MDPI
SN  - 20754418
KW  - non-invasive fetal genotyping
KW  - RHDgene
KW  - cell-free fetal DNA
KW  - real-time PCR
KW  - QF PCR
KW  - Rh blood group system
KW  - red blood cell alloimmunization
KW  - hemolytic disease of the fetus and newborn
UR  - https://www.mdpi.com/2075-4418/10/8/564
L2  - https://www.mdpi.com/2075-4418/10/8/564
N2  - Noninvasive fetalRHDgenotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetalRHDgenotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetalRHDgenotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from theRHDgene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of theRHDgenotyping. TheRHDgenotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict theRHDgenotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetalRHDallele from the plasma of RhD-negative pregnant women.
ER  -

BOHMOVA, Jana, Marek LUBUSKY, Iva HOLUSKOVA, Martina STUDNICKOVA, Romana KRATOCHVILOVA, Eva KREJCIRIKOVA, Veronika DURDOVA, Tereza KRATOCHVILOVA, Ladislav DUŠEK, Martin PROCHAZKA and Radek VODICKA. Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma. \textit{Diagnostics}. Basel: MDPI, 2020, vol.~10, No~8, p.~1-15. ISSN~2075-4418. Available from: https://dx.doi.org/10.3390/diagnostics10080564.

Detailed Information on Publication Record