Biosensors – Where are the Limits

a 2020

Biosensors – Where are the Limits

SKLÁDAL, Petr; Zdeněk FARKA; Matěj PASTUCHA; Radka OBOŘILOVÁ; Karel LACINA et. al.

Základní údaje

Originální název

Biosensors – Where are the Limits

Autoři

SKLÁDAL, Petr; Zdeněk FARKA; Matěj PASTUCHA; Radka OBOŘILOVÁ; Karel LACINA; Jan PŘIBYL a Guido CALUORI

Vydání

XX. Workshop of Biophysical Chemists and Electrochemists, 2020

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-9655-4

Změněno: 24. 9. 2020 17:53, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

The ability to detect very low concentrations of analytes is of fundamental importance. In many critical situations, even few molecules of toxins can be harmful, individual pathogenic microbes initiate infectious disease, and traces of cancer markers indicate beginning of malignant transformation. From the suitable approaches, single-molecule immunochemical sensors and assays provide the detection of individual analyte molecules reaching the ultimate sensitivity in analytical chemistry. Immunosensors are being developed to enhance the sensing capabilities, lower limits of detection and provide point-of-care mode of use with short analysis times. The promising approaches recently initiated in our laboratory include digital affinity sensing - single-molecule assays (or better single-analyte to include also viruses, bacteria and cells), combination of optical and electrochemical transduction (electrochemiluminescence, photoelectrochemical conversion), and inorganic nanocrystals for either luminescent labelling (quantum dots, photon up-conversion nanoparticles) or catalytic (Prussian blue) signal enhancement. Furthermore, advanced nanotools based on scanning probe microscopies allowed to touch and interface individual cells using nanomechanical biosensors, in combination with microelectrode arrays and fluorescence. This provides detailed information on the physiological state of patients-derived cells, allows to model disease progress at the cellular level and optimize medication leading to personalized medicine. In conclusion, the combination of biosensors with new tools and materials resulting from nanobiotechnology studies allowed to direct this field to new horizons.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

SKLÁDAL, Petr; Zdeněk FARKA; Matěj PASTUCHA; Radka OBOŘILOVÁ; Karel LACINA; Jan PŘIBYL a Guido CALUORI. Biosensors – Where are the Limits. In XX. Workshop of Biophysical Chemists and Electrochemists. 2020. ISBN 978-80-210-9655-4.

@proceedings{1680556,
   author = {Skládal, Petr and Farka, Zdeněk and Pastucha, Matěj and Obořilová, Radka and Lacina, Karel and Přibyl, Jan and Caluori, Guido},
   booktitle = {XX. Workshop of Biophysical Chemists and Electrochemists},
   language = {eng},
   isbn = {978-80-210-9655-4},
   title = {Biosensors – Where are the Limits},
   url = {https://www.sci.muni.cz/labifel/files/soubory/sbornik_2020.pdf#page=11},
   year = {2020}
}

TY  - CONF
ID  - 1680556
AU  - Skládal, Petr - Farka, Zdeněk - Pastucha, Matěj - Obořilová, Radka - Lacina, Karel - Přibyl, Jan - Caluori, Guido
PY  - 2020
TI  - Biosensors – Where are the Limits
SN  - 9788021096554
UR  - https://www.sci.muni.cz/labifel/files/soubory/sbornik_2020.pdf#page=11
N2  - The ability to detect very low concentrations of analytes is of fundamental importance. In many critical situations, even few molecules of toxins can be harmful, individual pathogenic microbes initiate infectious disease, and traces of cancer markers indicate beginning of malignant transformation. From the suitable approaches, single-molecule immunochemical sensors and assays provide the detection of individual analyte molecules reaching the ultimate sensitivity in analytical chemistry. Immunosensors are being developed to enhance the sensing capabilities, lower limits of detection and provide point-of-care mode of use with short analysis times. The promising approaches recently initiated in our laboratory include digital affinity sensing - single-molecule assays (or better single-analyte to include also viruses, bacteria and cells), combination of optical and electrochemical transduction (electrochemiluminescence, photoelectrochemical conversion), and inorganic nanocrystals for either luminescent labelling (quantum dots, photon up-conversion nanoparticles) or catalytic (Prussian blue) signal enhancement. Furthermore, advanced nanotools based on scanning probe microscopies allowed to touch and interface individual cells using nanomechanical biosensors, in combination with microelectrode arrays and fluorescence. This provides detailed information on the physiological state of patients-derived cells, allows to model disease progress at the cellular level and optimize medication leading to personalized medicine. In conclusion, the combination of biosensors with new tools and materials resulting from nanobiotechnology studies allowed to direct this field to new horizons.
ER  -

SKLÁDAL, Petr; Zdeněk FARKA; Matěj PASTUCHA; Radka OBOŘILOVÁ; Karel LACINA; Jan PŘIBYL a Guido CALUORI. Biosensors – Where are the Limits. In \textit{XX. Workshop of Biophysical Chemists and Electrochemists}. 2020. ISBN~978-80-210-9655-4.

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