Biosensors – Where are the Limits

a 2020

Biosensors – Where are the Limits

SKLÁDAL, Petr, Zdeněk FARKA, Matěj PASTUCHA, Radka OBOŘILOVÁ, Karel LACINA et. al.

Basic information

Original name

Biosensors – Where are the Limits

Authors

SKLÁDAL, Petr, Zdeněk FARKA, Matěj PASTUCHA, Radka OBOŘILOVÁ, Karel LACINA, Jan PŘIBYL and Guido CALUORI

Edition

XX. Workshop of Biophysical Chemists and Electrochemists, 2020

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Science

ISBN

978-80-210-9655-4

Změněno: 24/9/2020 17:53, doc. Mgr. Zdeněk Farka, Ph.D.

Abstract

V originále

The ability to detect very low concentrations of analytes is of fundamental importance. In many critical situations, even few molecules of toxins can be harmful, individual pathogenic microbes initiate infectious disease, and traces of cancer markers indicate beginning of malignant transformation. From the suitable approaches, single-molecule immunochemical sensors and assays provide the detection of individual analyte molecules reaching the ultimate sensitivity in analytical chemistry. Immunosensors are being developed to enhance the sensing capabilities, lower limits of detection and provide point-of-care mode of use with short analysis times. The promising approaches recently initiated in our laboratory include digital affinity sensing - single-molecule assays (or better single-analyte to include also viruses, bacteria and cells), combination of optical and electrochemical transduction (electrochemiluminescence, photoelectrochemical conversion), and inorganic nanocrystals for either luminescent labelling (quantum dots, photon up-conversion nanoparticles) or catalytic (Prussian blue) signal enhancement. Furthermore, advanced nanotools based on scanning probe microscopies allowed to touch and interface individual cells using nanomechanical biosensors, in combination with microelectrode arrays and fluorescence. This provides detailed information on the physiological state of patients-derived cells, allows to model disease progress at the cellular level and optimize medication leading to personalized medicine. In conclusion, the combination of biosensors with new tools and materials resulting from nanobiotechnology studies allowed to direct this field to new horizons.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SKLÁDAL, Petr, Zdeněk FARKA, Matěj PASTUCHA, Radka OBOŘILOVÁ, Karel LACINA, Jan PŘIBYL and Guido CALUORI. Biosensors – Where are the Limits. In XX. Workshop of Biophysical Chemists and Electrochemists. 2020. ISBN 978-80-210-9655-4.

@proceedings{1680556,
   author = {Skládal, Petr and Farka, Zdeněk and Pastucha, Matěj and Obořilová, Radka and Lacina, Karel and Přibyl, Jan and Caluori, Guido},
   booktitle = {XX. Workshop of Biophysical Chemists and Electrochemists},
   language = {eng},
   isbn = {978-80-210-9655-4},
   title = {Biosensors – Where are the Limits},
   url = {https://www.sci.muni.cz/labifel/files/soubory/sbornik_2020.pdf#page=11},
   year = {2020}
}

TY  - CONF
ID  - 1680556
AU  - Skládal, Petr - Farka, Zdeněk - Pastucha, Matěj - Obořilová, Radka - Lacina, Karel - Přibyl, Jan - Caluori, Guido
PY  - 2020
TI  - Biosensors – Where are the Limits
SN  - 9788021096554
UR  - https://www.sci.muni.cz/labifel/files/soubory/sbornik_2020.pdf#page=11
N2  - The ability to detect very low concentrations of analytes is of fundamental importance. In many critical situations, even few molecules of toxins can be harmful, individual pathogenic microbes initiate infectious disease, and traces of cancer markers indicate beginning of malignant transformation. From the suitable approaches, single-molecule immunochemical sensors and assays provide the detection of individual analyte molecules reaching the ultimate sensitivity in analytical chemistry. Immunosensors are being developed to enhance the sensing capabilities, lower limits of detection and provide point-of-care mode of use with short analysis times. The promising approaches recently initiated in our laboratory include digital affinity sensing - single-molecule assays (or better single-analyte to include also viruses, bacteria and cells), combination of optical and electrochemical transduction (electrochemiluminescence, photoelectrochemical conversion), and inorganic nanocrystals for either luminescent labelling (quantum dots, photon up-conversion nanoparticles) or catalytic (Prussian blue) signal enhancement. Furthermore, advanced nanotools based on scanning probe microscopies allowed to touch and interface individual cells using nanomechanical biosensors, in combination with microelectrode arrays and fluorescence. This provides detailed information on the physiological state of patients-derived cells, allows to model disease progress at the cellular level and optimize medication leading to personalized medicine. In conclusion, the combination of biosensors with new tools and materials resulting from nanobiotechnology studies allowed to direct this field to new horizons.
ER  -

SKLÁDAL, Petr, Zdeněk FARKA, Matěj PASTUCHA, Radka OBOŘILOVÁ, Karel LACINA, Jan PŘIBYL and Guido CALUORI. Biosensors – Where are the Limits. In \textit{XX. Workshop of Biophysical Chemists and Electrochemists}. 2020. ISBN~978-80-210-9655-4.

Detailed Information on Publication Record