miR-183/96/182 cluster is an important morphogenetic factor
targetingPAX6expression in differentiating human retinal
organoids

J 2020

miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids

PEŠKOVÁ, Lucie, Denisa JURČÍKOVÁ, Tereza VÁŇOVÁ, Jan KŘIVÁNEK, Michaela CAPANDOVÁ et. al.

Basic information

Original name

miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids

Authors

PEŠKOVÁ, Lucie (203 Czech Republic, belonging to the institution), Denisa JURČÍKOVÁ (203 Czech Republic, belonging to the institution), Tereza VÁŇOVÁ (203 Czech Republic, belonging to the institution), Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution), Michaela CAPANDOVÁ (203 Czech Republic, belonging to the institution), Zuzana ŠRÁMKOVÁ (703 Slovakia, belonging to the institution), Jana ŠEBESTÍKOVÁ (203 Czech Republic, belonging to the institution), Magdaléna KOLOUŠKOVÁ (203 Czech Republic, belonging to the institution), Hana KOTASOVÁ (203 Czech Republic, belonging to the institution), Libor STREIT (203 Czech Republic) and Tomáš BÁRTA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Stem Cells, Hoboken, Wiley-Blackwell, 2020, 1066-5099

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.277

RIV identification code

RIV/00216224:14110/20:00116545

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/stem.3272

UT WoS

000569493800001

Keywords in English

microRNAs; organoids; pluripotent stem cells; retina

Abstract

V originále

MicroRNAs (miRNAs), a class of small, noncoding RNA molecules represent important regulators of gene expression. Recent reports have implicated their role in the cell specification process acting as "fine-tuners" to ensure the precise gene expression at the specific stage of cell differentiation. Here, we used retinal organoids differentiated from human pluripotent stem cells (hPSCs) as a model to closely investigate the role of a sensory organ-specific and evolutionary conserved miR-183/96/182 cluster. Using a miRNA tough decoy approach, we inhibited the miR-183/96/182 cluster in hPSCs. Inhibition of the miRNA cluster resulted in an increased expansion of neuroepithelium leading to abnormal "bulged" neural retina in organoids, associated with upregulation of neural-specific and retinal-specific genes. Importantly, we identifiedPAX6, a well-known essential gene in neuroectoderm specification, as a target of the miR-183/96/182 cluster members. Taken together, the miR-183/96/182 cluster not only represents an important regulator ofPAX6expression, but it also plays a crucial role in retinal tissue morphogenesis.

Links

MUNI/G/1391/2018, interní kód MU

Name: At the Cross-road of Magnetic Fields and Light: A New Perspective of Cell Clock Control.

Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects

ROZV/28/LF20/2020, interní kód MU

Name: Využiti retinálních organoidů pro studium vlivu fotostimulace na lidskou retinu

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

PEŠKOVÁ, Lucie, Denisa JURČÍKOVÁ, Tereza VÁŇOVÁ, Jan KŘIVÁNEK, Michaela CAPANDOVÁ, Zuzana ŠRÁMKOVÁ, Jana ŠEBESTÍKOVÁ, Magdaléna KOLOUŠKOVÁ, Hana KOTASOVÁ, Libor STREIT and Tomáš BÁRTA. miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids. Stem Cells. Hoboken: Wiley-Blackwell, 2020, vol. 38, No 12, p. 1557-1567. ISSN 1066-5099. Available from: https://dx.doi.org/10.1002/stem.3272.

@article{1682016,
   author = {Pešková, Lucie and Jurčíková, Denisa and Váňová, Tereza and Křivánek, Jan and Capandová, Michaela and Šrámková, Zuzana and Šebestíková, Jana and Koloušková, Magdaléna and Kotasová, Hana and Streit, Libor and Bárta, Tomáš},
   article_location = {Hoboken},
   article_number = {12},
   doi = {http://dx.doi.org/10.1002/stem.3272},
   keywords = {microRNAs; organoids; pluripotent stem cells; retina},
   language = {eng},
   issn = {1066-5099},
   journal = {Stem Cells},
   title = {miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids},
   url = {https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272},
   volume = {38},
   year = {2020}
}

TY  - JOUR
ID  - 1682016
AU  - Pešková, Lucie - Jurčíková, Denisa - Váňová, Tereza - Křivánek, Jan - Capandová, Michaela - Šrámková, Zuzana - Šebestíková, Jana - Koloušková, Magdaléna - Kotasová, Hana - Streit, Libor - Bárta, Tomáš
PY  - 2020
TI  - miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids
JF  - Stem Cells
VL  - 38
IS  - 12
SP  - 1557-1567
EP  - 1557-1567
PB  - Wiley-Blackwell
SN  - 10665099
KW  - microRNAs
KW  - organoids
KW  - pluripotent stem cells
KW  - retina
UR  - https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272
L2  - https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272
N2  - MicroRNAs (miRNAs), a class of small, noncoding RNA molecules represent important regulators of gene expression. Recent reports have implicated their role in the cell specification process acting as "fine-tuners" to ensure the precise gene expression at the specific stage of cell differentiation. Here, we used retinal organoids differentiated from human pluripotent stem cells (hPSCs) as a model to closely investigate the role of a sensory organ-specific and evolutionary conserved miR-183/96/182 cluster. Using a miRNA tough decoy approach, we inhibited the miR-183/96/182 cluster in hPSCs. Inhibition of the miRNA cluster resulted in an increased expansion of neuroepithelium leading to abnormal "bulged" neural retina in organoids, associated with upregulation of neural-specific and retinal-specific genes. Importantly, we identifiedPAX6, a well-known essential gene in neuroectoderm specification, as a target of the miR-183/96/182 cluster members. Taken together, the miR-183/96/182 cluster not only represents an important regulator ofPAX6expression, but it also plays a crucial role in retinal tissue morphogenesis.
ER  -

PEŠKOVÁ, Lucie, Denisa JURČÍKOVÁ, Tereza VÁŇOVÁ, Jan KŘIVÁNEK, Michaela CAPANDOVÁ, Zuzana ŠRÁMKOVÁ, Jana ŠEBESTÍKOVÁ, Magdaléna KOLOUŠKOVÁ, Hana KOTASOVÁ, Libor STREIT and Tomáš BÁRTA. miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids. \textit{Stem Cells}. Hoboken: Wiley-Blackwell, 2020, vol.~38, No~12, p.~1557-1567. ISSN~1066-5099. Available from: https://dx.doi.org/10.1002/stem.3272.

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