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@article{1682016,
author = {Pešková, Lucie and Jurčíková, Denisa and Váňová, Tereza and Křivánek, Jan and Capandová, Michaela and Šrámková, Zuzana and Šebestíková, Jana and Koloušková, Magdaléna and Kotasová, Hana and Streit, Libor and Bárta, Tomáš},
article_location = {Hoboken},
article_number = {12},
doi = {http://dx.doi.org/10.1002/stem.3272},
keywords = {microRNAs; organoids; pluripotent stem cells; retina},
language = {eng},
issn = {1066-5099},
journal = {Stem Cells},
title = {miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids},
url = {https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272},
volume = {38},
year = {2020}
}
TY - JOUR
ID - 1682016
AU - Pešková, Lucie - Jurčíková, Denisa - Váňová, Tereza - Křivánek, Jan - Capandová, Michaela - Šrámková, Zuzana - Šebestíková, Jana - Koloušková, Magdaléna - Kotasová, Hana - Streit, Libor - Bárta, Tomáš
PY - 2020
TI - miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids
JF - Stem Cells
VL - 38
IS - 12
SP - 1557-1567
EP - 1557-1567
PB - Wiley-Blackwell
SN - 10665099
KW - microRNAs
KW - organoids
KW - pluripotent stem cells
KW - retina
UR - https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272
L2 - https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272
N2 - MicroRNAs (miRNAs), a class of small, noncoding RNA molecules represent important regulators of gene expression. Recent reports have implicated their role in the cell specification process acting as "fine-tuners" to ensure the precise gene expression at the specific stage of cell differentiation. Here, we used retinal organoids differentiated from human pluripotent stem cells (hPSCs) as a model to closely investigate the role of a sensory organ-specific and evolutionary conserved miR-183/96/182 cluster. Using a miRNA tough decoy approach, we inhibited the miR-183/96/182 cluster in hPSCs. Inhibition of the miRNA cluster resulted in an increased expansion of neuroepithelium leading to abnormal "bulged" neural retina in organoids, associated with upregulation of neural-specific and retinal-specific genes. Importantly, we identifiedPAX6, a well-known essential gene in neuroectoderm specification, as a target of the miR-183/96/182 cluster members. Taken together, the miR-183/96/182 cluster not only represents an important regulator ofPAX6expression, but it also plays a crucial role in retinal tissue morphogenesis.
ER -
PEŠKOVÁ, Lucie, Denisa JURČÍKOVÁ, Tereza VÁŇOVÁ, Jan KŘIVÁNEK, Michaela CAPANDOVÁ, Zuzana ŠRÁMKOVÁ, Jana ŠEBESTÍKOVÁ, Magdaléna KOLOUŠKOVÁ, Hana KOTASOVÁ, Libor STREIT and Tomáš BÁRTA. miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids. \textit{Stem Cells}. Hoboken: Wiley-Blackwell, 2020, vol.~38, No~12, p.~1557-1567. ISSN~1066-5099. Available from: https://dx.doi.org/10.1002/stem.3272.
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