Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines

DVOŘÁKOVÁ, Monika, Petr LAPČÍK, Pavla BOUCHALOVÁ and Pavel BOUCHAL. Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines. Proteomics. Hoboken: Wiley, vol. 20, No 7, p. 1-8. ISSN 1615-9853. doi:10.1002/pmic.201900383. 2020.

Basic information

• Original name: Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines
• Authors: DVOŘÁKOVÁ, Monika (203 Czech Republic, belonging to the institution), Petr LAPČÍK (203 Czech Republic, belonging to the institution), Pavla BOUCHALOVÁ (203 Czech Republic, belonging to the institution) and Pavel BOUCHAL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Proteomics, Hoboken, Wiley, 2020, 1615-9853.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.984
• RIV identification code: RIV/00216224:14310/20:00114343
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1002/pmic.201900383
• UT WoS: 000525195800001
• Keywords in English: apoptosis; breast cancer; cytoskeleton; metastasis; migration; transgelin
• Tags: CF PROT
• Tags: International impact, Reviewed

Abstract

Transgelin is a protein reported to be a marker of several cancers. However, previous studies have shown both up- and down-regulation of transgelin in tumors when compared with non-tumor tissues and the mechanisms whereby transgelin may affect the development of cancer remain largely unknown. Transgelin is especially abundant in smooth muscle cells and is associated with actin stress fibers. These contractile structures participate in cell motility, adhesion, and the maintenance of cell morphology. Here, the role of transgelin in breast cancer is focused on. Initially, the effects of transgelin on cell migration of the breast cancer cell lines, BT 549 and PMC 42, is studied. Interestingly, transgelin silencing increased the migration of PMC 42 cells, but decreased the migration of BT 549 cells. To clarify these contradictory results, the changes in protein abundances after transgelin silencing in these two cell lines are analyzed using quantitative proteomics. The results confirmed the role of transgelin in the migration of BT 549 cells and suggest the involvement of transgelin in apoptosis and small molecule biochemistry in PMC 42 cells. The context-dependent function of transgelin reflects the different molecular backgrounds of these cell lines, which differ in karyotypes, mutation statuses, and proteome profiles.

Links

• GA17-05957S, research and development project: Name: Evaluace nových potenciálních cílů a inhibitorů pro blokování vývoje metastáz u luminálních A nádorů prsu

Investor: Czech Science Foundation

• LM2015043, research and development project: Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

• MUNI/A/1252/2019, interní kód MU: Name: Podpora biochemického výzkumu v roce 2020

Investor: Masaryk University, Category A

• NV19-08-00250, research and development project: Name: Proteotypová klasifikace renálního karcinomu ve vztahu k prognóze a terapeutické odpovědi

Investor: Ministry of Health of the CR