Transgelin Silencing Induces Different Processes in Different
Breast Cancer Cell Lines

J 2020

Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines

DVOŘÁKOVÁ, Monika, Petr LAPČÍK, Pavla BOUCHALOVÁ and Pavel BOUCHAL

Basic information

Original name

Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines

Authors

DVOŘÁKOVÁ, Monika (203 Czech Republic, belonging to the institution), Petr LAPČÍK (203 Czech Republic, belonging to the institution), Pavla BOUCHALOVÁ (203 Czech Republic, belonging to the institution) and Pavel BOUCHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Proteomics, Hoboken, Wiley, 2020, 1615-9853

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.984

RIV identification code

RIV/00216224:14310/20:00114343

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/pmic.201900383

UT WoS

000525195800001

Keywords in English

apoptosis; breast cancer; cytoskeleton; metastasis; migration; transgelin

Abstract

V originále

Transgelin is a protein reported to be a marker of several cancers. However, previous studies have shown both up- and down-regulation of transgelin in tumors when compared with non-tumor tissues and the mechanisms whereby transgelin may affect the development of cancer remain largely unknown. Transgelin is especially abundant in smooth muscle cells and is associated with actin stress fibers. These contractile structures participate in cell motility, adhesion, and the maintenance of cell morphology. Here, the role of transgelin in breast cancer is focused on. Initially, the effects of transgelin on cell migration of the breast cancer cell lines, BT 549 and PMC 42, is studied. Interestingly, transgelin silencing increased the migration of PMC 42 cells, but decreased the migration of BT 549 cells. To clarify these contradictory results, the changes in protein abundances after transgelin silencing in these two cell lines are analyzed using quantitative proteomics. The results confirmed the role of transgelin in the migration of BT 549 cells and suggest the involvement of transgelin in apoptosis and small molecule biochemistry in PMC 42 cells. The context-dependent function of transgelin reflects the different molecular backgrounds of these cell lines, which differ in karyotypes, mutation statuses, and proteome profiles.

Links

GA17-05957S, research and development project

Name: Evaluace nových potenciálních cílů a inhibitorů pro blokování vývoje metastáz u luminálních A nádorů prsu

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1252/2019, interní kód MU

Name: Podpora biochemického výzkumu v roce 2020

Investor: Masaryk University, Category A

NV19-08-00250, research and development project

Name: Proteotypová klasifikace renálního karcinomu ve vztahu k prognóze a terapeutické odpovědi

Investor: Ministry of Health of the CR

Citovat

DVOŘÁKOVÁ, Monika, Petr LAPČÍK, Pavla BOUCHALOVÁ and Pavel BOUCHAL. Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines. Proteomics. Hoboken: Wiley, 2020, vol. 20, No 7, p. 1-8. ISSN 1615-9853. Available from: https://dx.doi.org/10.1002/pmic.201900383.

@article{1682960,
   author = {Dvořáková, Monika and Lapčík, Petr and Bouchalová, Pavla and Bouchal, Pavel},
   article_location = {Hoboken},
   article_number = {7},
   doi = {http://dx.doi.org/10.1002/pmic.201900383},
   keywords = {apoptosis; breast cancer; cytoskeleton; metastasis; migration; transgelin},
   language = {eng},
   issn = {1615-9853},
   journal = {Proteomics},
   title = {Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines},
   url = {https://doi.org/10.1002/pmic.201900383},
   volume = {20},
   year = {2020}
}

TY  - JOUR
ID  - 1682960
AU  - Dvořáková, Monika - Lapčík, Petr - Bouchalová, Pavla - Bouchal, Pavel
PY  - 2020
TI  - Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines
JF  - Proteomics
VL  - 20
IS  - 7
SP  - 1-8
EP  - 1-8
PB  - Wiley
SN  - 16159853
KW  - apoptosis
KW  - breast cancer
KW  - cytoskeleton
KW  - metastasis
KW  - migration
KW  - transgelin
UR  - https://doi.org/10.1002/pmic.201900383
L2  - https://doi.org/10.1002/pmic.201900383
N2  - Transgelin is a protein reported to be a marker of several cancers. However, previous studies have shown both up- and down-regulation of transgelin in tumors when compared with non-tumor tissues and the mechanisms whereby transgelin may affect the development of cancer remain largely unknown. Transgelin is especially abundant in smooth muscle cells and is associated with actin stress fibers. These contractile structures participate in cell motility, adhesion, and the maintenance of cell morphology. Here, the role of transgelin in breast cancer is focused on. Initially, the effects of transgelin on cell migration of the breast cancer cell lines, BT 549 and PMC 42, is studied. Interestingly, transgelin silencing increased the migration of PMC 42 cells, but decreased the migration of BT 549 cells. To clarify these contradictory results, the changes in protein abundances after transgelin silencing in these two cell lines are analyzed using quantitative proteomics. The results confirmed the role of transgelin in the migration of BT 549 cells and suggest the involvement of transgelin in apoptosis and small molecule biochemistry in PMC 42 cells. The context-dependent function of transgelin reflects the different molecular backgrounds of these cell lines, which differ in karyotypes, mutation statuses, and proteome profiles.
ER  -

DVOŘÁKOVÁ, Monika, Petr LAPČÍK, Pavla BOUCHALOVÁ and Pavel BOUCHAL. Transgelin Silencing Induces Different Processes in Different Breast Cancer Cell Lines. \textit{Proteomics}. Hoboken: Wiley, 2020, vol.~20, No~7, p.~1-8. ISSN~1615-9853. Available from: https://dx.doi.org/10.1002/pmic.201900383.

Detailed Information on Publication Record