| ŠVEC, Pavel, Marcel KOSINA, Michal ZEMAN, Pavla HOLOCHOVÁ, Stanislava KRÁLOVÁ, Eva NĚMCOVÁ, Lenka MICENKOVÁ, Urvashi SINGH B., Vipin GUPTA, Utkarsh SOOD, Rup LAL, Suresh KORPOLE and Ivo SEDLÁČEK. Pseudomonas karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves. International Journal of Systematic and Evolutionary Microbiology. London: Microbiology Society, 2020, vol. 70, No 9, p. 5131-5140. ISSN 1466-5026. Available from: https://dx.doi.org/10.1099/ijsem.0.004393. |
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@article{1683556,
author = {Švec, Pavel and Kosina, Marcel and Zeman, Michal and Holochová, Pavla and Králová, Stanislava and Němcová, Eva and Micenková, Lenka and Singh B., Urvashi and Gupta, Vipin and Sood, Utkarsh and Lal, Rup and Korpole, Suresh and Sedláček, Ivo},
article_location = {London},
article_number = {9},
doi = {http://dx.doi.org/10.1099/ijsem.0.004393},
keywords = {caves; moonmilk; Pseudomonas karstica; Pseudomonas spelaei and sp. nov.},
language = {eng},
issn = {1466-5026},
journal = {International Journal of Systematic and Evolutionary Microbiology},
note = {CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat. Místo toho uplatnit EF17_043/0009632.},
title = {Pseudomonas karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves},
url = {https://doi.org/10.1099/ijsem.0.004393},
volume = {70},
year = {2020}
}
TY - JOUR
ID - 1683556
AU - Švec, Pavel - Kosina, Marcel - Zeman, Michal - Holochová, Pavla - Králová, Stanislava - Němcová, Eva - Micenková, Lenka - Singh B., Urvashi - Gupta, Vipin - Sood, Utkarsh - Lal, Rup - Korpole, Suresh - Sedláček, Ivo
PY - 2020
TI - Pseudomonas karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves
JF - International Journal of Systematic and Evolutionary Microbiology
VL - 70
IS - 9
SP - 5131-5140
EP - 5131-5140
PB - Microbiology Society
SN - 14665026
N1 - CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat. Místo toho uplatnit EF17_043/0009632.
KW - caves
KW - moonmilk
KW - Pseudomonas karstica
KW - Pseudomonas spelaei and sp. nov.
UR - https://doi.org/10.1099/ijsem.0.004393
L2 - https://doi.org/10.1099/ijsem.0.004393
N2 - A taxonomic study of two fluorescent Pseudomonas strains (HJ/4(T) and SJ/9/1(T)) isolated from calcite moonmilk samples obtained from two caves in the Moravian Karst in the Czech Republic was carried out. Results of initial 16S rRNA gene sequence analysis assigned both strains into the genus Pseudomonas and showed Pseudomonas yamanorum 8H1(T) as their closest neighbour with 99.8 and 99.7 % 16S rRNA gene similarities to strains HJ/4(T) and SJ/9/1(T). respectively. Subsequent sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of both isolates to P. yamanorum 8H1(T). but phylogeny and sequences similarities implied that they are representatives of two novel species within the genus Pseudomonas. Further study comprising whole-genome sequencing followed by average nucleotide identity and digital DNA-DNA hybridization calculations, repetitive sequence-based PCR fingerprinting with the REP and ERIC primers, automated ribotyping with the EcoRl restriction endonuclease, cellular fatty acid analysis, quinone and polar lipid characterization, and extensive biotyping confirmed clear separation of both analysed strains from the remaining Pseudomonas species and showed that they represent two novel species within the genus Pseudomonas for which the names Pseudomonas karstica sp. nov. (type strain HJ/4(T) =CCM 7891(T) =LMG 27930(T)) and Pseudomonas spelaei sp. nov. (type strain SJ/9/1(T)=CCM 7893(T)=LMG 27931(T)) are suggested.
ER -
ŠVEC, Pavel, Marcel KOSINA, Michal ZEMAN, Pavla HOLOCHOVÁ, Stanislava KRÁLOVÁ, Eva NĚMCOVÁ, Lenka MICENKOVÁ, Urvashi SINGH B., Vipin GUPTA, Utkarsh SOOD, Rup LAL, Suresh KORPOLE and Ivo SEDLÁČEK. Pseudomonas karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves. \textit{International Journal of Systematic and Evolutionary Microbiology}. London: Microbiology Society, 2020, vol.~70, No~9, p.~5131-5140. ISSN~1466-5026. Available from: https://dx.doi.org/10.1099/ijsem.0.004393.
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