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@article{1684416,
author = {Pilat, Zdenek and Jonas, Alexandr and Pilatova, Jana and Klementova, Tereza and Bernatova, Silvie and Siler, Martin and Manka, Tadeas and Kizovsky, Martin and Růžička, Filip and Pantůček, Roman and Neugebauer, Ute and Samek, Ota and Zemanek, Pavel},
article_location = {Washington},
article_number = {18},
doi = {http://dx.doi.org/10.1021/acs.analchem.0c01963},
keywords = {Infectious diseases; Viruses; Bioinorganic chemistry; Genetics; Raman spectroscopy},
language = {eng},
issn = {0003-2700},
journal = {Analytical chemistry},
title = {Analysis of Bacteriophage-Host Interaction by Raman Tweezers},
url = {https://doi.org/10.1021/acs.analchem.0c01963},
volume = {92},
year = {2020}
}
TY - JOUR
ID - 1684416
AU - Pilat, Zdenek - Jonas, Alexandr - Pilatova, Jana - Klementova, Tereza - Bernatova, Silvie - Siler, Martin - Manka, Tadeas - Kizovsky, Martin - Růžička, Filip - Pantůček, Roman - Neugebauer, Ute - Samek, Ota - Zemanek, Pavel
PY - 2020
TI - Analysis of Bacteriophage-Host Interaction by Raman Tweezers
JF - Analytical chemistry
VL - 92
IS - 18
SP - 12304-12311
EP - 12304-12311
PB - American Chemical Society
SN - 00032700
KW - Infectious diseases
KW - Viruses
KW - Bioinorganic chemistry
KW - Genetics
KW - Raman spectroscopy
UR - https://doi.org/10.1021/acs.analchem.0c01963
L2 - https://doi.org/10.1021/acs.analchem.0c01963
N2 - Bacteriophages, or "phages" for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.
ER -
PILAT, Zdenek, Alexandr JONAS, Jana PILATOVA, Tereza KLEMENTOVA, Silvie BERNATOVA, Martin SILER, Tadeas MANKA, Martin KIZOVSKY, Filip RŮŽIČKA, Roman PANTŮČEK, Ute NEUGEBAUER, Ota SAMEK and Pavel ZEMANEK. Analysis of Bacteriophage-Host Interaction by Raman Tweezers. \textit{Analytical chemistry}. Washington: American Chemical Society, 2020, vol.~92, No~18, p.~12304-12311. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.0c01963.
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