J 2020

Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone

PELTOMAA, Riikka, Zdeněk FARKA, Matthias Jürgen MICKERT, Julian BRANDMEIER, Matěj PASTUCHA et. al.

Basic information

Original name

Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone

Authors

PELTOMAA, Riikka (246 Finland), Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution), Matthias Jürgen MICKERT (276 Germany), Julian BRANDMEIER (276 Germany), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Mónica MARTÍNEZ-ORTS (724 Spain), Ángeles CANALES (724 Spain), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Elena BENITO-PEÑA (724 Spain), María C. MORENO-BONDI (724 Spain) and Hans-Heiner GORRIS (276 Germany)

Edition

Biosensors and Bioelectronics, Oxford, Elsevier B.V. 2020, 0956-5663

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 10.618

RIV identification code

RIV/00216224:14310/20:00116649

Organization unit

Faculty of Science

DOI

UT WoS

000596868900004

Keywords in English

Zearalenone; Upconversion nanoparticle; Peptide mimetic; Immunosensing; Surface plasmon resonance; Food safety

Tags

Tags

International impact, Reviewed
Změněno: 24/10/2024 13:00, Mgr. Adéla Pešková

Abstract

V originále

Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) for the analysis of zearalenone (ZEA), one of the most frequently encountered mycotoxins in food worldwide. Instead of a toxin-conjugate conventionally used in competitive immunoassays, we designed a ZEA mimicking peptide extended by a biotin-linker and confirmed its excellent suitability to mimic ZEA by nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analysis. Upconversion nanoparticles (UCNP, type NaYF4:Yb,Tm) served as background-free optical label for the detection of the peptide mimetic in the competitive ULISA. Streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. The UCNP conjugate clearly outperformed conventional labels such as enzymes or fluorescent dyes. With a limit of detection of 20 pg mL-1 (63 pM), the competitive ULISA is well applicable to the detection of ZEA at the levels set by the European legislation. Moreover, the ULISA is specific for ZEA and its metabolites (alpha- and beta-zearalenol) without significant cross-reactivity with other related mycotoxins. We detected ZEA in spiked and naturally contaminated maize samples using liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) as a reference method to demonstrate food analysis in real samples.

Links

LM2018127, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
LTAB19011, research and development project
Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel
Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION
MUNI/A/1252/2019, interní kód MU
Name: Podpora biochemického výzkumu v roce 2020
Investor: Masaryk University, Category A