Ni and TiO2 nanoparticles cause adhesion and cytoskeletal
changes in human osteoblasts

J 2021

Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts

ŠTEFANČÍK, Michal, Lucie VÁLKOVÁ, Jana VEVERKOVÁ, Jan BALVAN, Tomáš VIČAR et. al.

Basic information

Original name

Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts

Authors

ŠTEFANČÍK, Michal (703 Slovakia, belonging to the institution), Lucie VÁLKOVÁ (203 Czech Republic, belonging to the institution), Jana VEVERKOVÁ (203 Czech Republic, belonging to the institution), Jan BALVAN (203 Czech Republic, belonging to the institution), Tomáš VIČAR (203 Czech Republic, belonging to the institution), Petr BABULA (203 Czech Republic, belonging to the institution), Josef MASEK (203 Czech Republic), Pavel KULICH and Monika PÁVKOVÁ GOLDBERGOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Environmental Science and Pollution Research, Heidelberg, Springer Heidelberg, 2021, 0944-1344

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.190

RIV identification code

RIV/00216224:14110/21:00120075

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s11356-020-10908-8

UT WoS

000572893700002

Keywords in English

Ni nanoparticles; TiO2 nanoparticles; Osteoblasts; Cytoskeleton; Adhesion; Bone

Abstract

V originále

Titanium-based alloys have established a crucial role in implantology. As material deteriorates overtime, nanoparticles of TiO2 and Ni are released. This study is focused on the impact of TiO2 and Ni nanoparticles with size of 100 nm on cytoskeletal and adhesive changes in human physiological and osteoarthritic osteoblasts. The impact of nanoparticles with concentration of 1.5 ng/mL on actin and tubulin expression and gene expression of FAK and ICAM-1 was studied. The cell size and actin expression of physiological osteoblasts decreased in presence of Ni nanoparticles, while TiO(2)nanoparticles caused increase in cell size and actin expression. Both cell lines expressed more FAK as a response to TiO(2)nanoparticles. ICAM-1 gene was overexpressed in both cell lines as a reaction to both types of nanoparticles. The presented study shows a crucial role of Ni and TiO2 nanoparticles in human osteoblast cytoskeletal and adhesive changes, especially connected with the osteoarthritic cells.

Links

MUNI/A/1453/2019, interní kód MU

Name: Příspěvek ke studiu molekulární, buněčné, tkáňové a systémové patofyziologie vybraných komplexních nemocí

Investor: Masaryk University, Category A

MUNI/A/1698/2020, interní kód MU

Name: Od molekulární, buněčné a tkáňové k systémové patofyziologii vybraných komplexních nemocí (Acronym: ComplexPF)

Investor: Masaryk University

NU20-08-00149, research and development project

Name: Multicentrické hodnocení hypersenzitivní reakce u pacientů indikovaných k totální náhradě kloubu včetně hodnocení důvodů reimplanace

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

ŠTEFANČÍK, Michal, Lucie VÁLKOVÁ, Jana VEVERKOVÁ, Jan BALVAN, Tomáš VIČAR, Petr BABULA, Josef MASEK, Pavel KULICH and Monika PÁVKOVÁ GOLDBERGOVÁ. Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts. Environmental Science and Pollution Research. Heidelberg: Springer Heidelberg, 2021, vol. 28, No 5, p. 6018-6029. ISSN 0944-1344. Available from: https://dx.doi.org/10.1007/s11356-020-10908-8.

@article{1685062,
   author = {Štefančík, Michal and Válková, Lucie and Veverková, Jana and Balvan, Jan and Vičar, Tomáš and Babula, Petr and Masek, Josef and Kulich, Pavel and Pávková Goldbergová, Monika},
   article_location = {Heidelberg},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s11356-020-10908-8},
   keywords = {Ni nanoparticles; TiO2 nanoparticles; Osteoblasts; Cytoskeleton; Adhesion; Bone},
   language = {eng},
   issn = {0944-1344},
   journal = {Environmental Science and Pollution Research},
   title = {Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts},
   url = {https://link.springer.com/article/10.1007/s11356-020-10908-8},
   volume = {28},
   year = {2021}
}

TY  - JOUR
ID  - 1685062
AU  - Štefančík, Michal - Válková, Lucie - Veverková, Jana - Balvan, Jan - Vičar, Tomáš - Babula, Petr - Masek, Josef - Kulich, Pavel - Pávková Goldbergová, Monika
PY  - 2021
TI  - Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts
JF  - Environmental Science and Pollution Research
VL  - 28
IS  - 5
SP  - 6018-6029
EP  - 6018-6029
PB  - Springer Heidelberg
SN  - 09441344
KW  - Ni nanoparticles
KW  - TiO2 nanoparticles
KW  - Osteoblasts
KW  - Cytoskeleton
KW  - Adhesion
KW  - Bone
UR  - https://link.springer.com/article/10.1007/s11356-020-10908-8
L2  - https://link.springer.com/article/10.1007/s11356-020-10908-8
N2  - Titanium-based alloys have established a crucial role in implantology. As material deteriorates overtime, nanoparticles of TiO2 and Ni are released. This study is focused on the impact of TiO2 and Ni nanoparticles with size of 100 nm on cytoskeletal and adhesive changes in human physiological and osteoarthritic osteoblasts. The impact of nanoparticles with concentration of 1.5 ng/mL on actin and tubulin expression and gene expression of FAK and ICAM-1 was studied. The cell size and actin expression of physiological osteoblasts decreased in presence of Ni nanoparticles, while TiO(2)nanoparticles caused increase in cell size and actin expression. Both cell lines expressed more FAK as a response to TiO(2)nanoparticles. ICAM-1 gene was overexpressed in both cell lines as a reaction to both types of nanoparticles. The presented study shows a crucial role of Ni and TiO2 nanoparticles in human osteoblast cytoskeletal and adhesive changes, especially connected with the osteoarthritic cells.
ER  -

ŠTEFANČÍK, Michal, Lucie VÁLKOVÁ, Jana VEVERKOVÁ, Jan BALVAN, Tomáš VIČAR, Petr BABULA, Josef MASEK, Pavel KULICH and Monika PÁVKOVÁ GOLDBERGOVÁ. Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts. \textit{Environmental Science and Pollution Research}. Heidelberg: Springer Heidelberg, 2021, vol.~28, No~5, p.~6018-6029. ISSN~0944-1344. Available from: https://dx.doi.org/10.1007/s11356-020-10908-8.

Detailed Information on Publication Record