Poly(ADP-ribose) glycohydrolase coordinates meiotic DNA
double-strand break induction and repair independent of its
catalytic activity

J 2020

Poly(ADP-ribose) glycohydrolase coordinates meiotic DNA double-strand break induction and repair independent of its catalytic activity

JANISIW, Eva, Marilina RAICES, Fabiola BALMIR, Luis F. PAULIN, Antoine BAUDRIMONT et. al.

Basic information

Original name

Poly(ADP-ribose) glycohydrolase coordinates meiotic DNA double-strand break induction and repair independent of its catalytic activity

Authors

JANISIW, Eva (40 Austria), Marilina RAICES (840 United States of America), Fabiola BALMIR (840 United States of America), Luis F. PAULIN (40 Austria), Antoine BAUDRIMONT (40 Austria), Arndt VON HAESELER (40 Austria), Judith L. YANOWITZ (840 United States of America), Verena JANTSCH (40 Austria) and Nicola SILVA (380 Italy, guarantor, belonging to the institution)

Edition

Nature Communications, London, Nature Publishing Group, 2020, 2041-1723

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 14.919

RIV identification code

RIV/00216224:14110/20:00114363

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41467-020-18693-1

UT WoS

000573737400001

Keywords in English

Poly(ADP-ribose) glycohydrolase; meiotic DNA double-strand break induction

Abstract

V originále

Poly(ADP-ribosyl)ation is a reversible post-translational modification synthetized by ADP-ribose transferases and removed by poly(ADP-ribose) glycohydrolase (PARG), which plays important roles in DNA damage repair. While well-studied in somatic tissues, much less is known about poly(ADP-ribosyl)ation in the germline, where DNA double-strand breaks are introduced by a regulated program and repaired by crossover recombination to establish a tether between homologous chromosomes. The interaction between the parental chromosomes is facilitated by meiotic specific adaptation of the chromosome axes and cohesins, and reinforced by the synaptonemal complex. Here, we uncover an unexpected role for PARG in coordinating the induction of meiotic DNA breaks and their homologous recombination-mediated repair in Caenorhabditis elegans. PARG-1/PARG interacts with both axial and central elements of the synaptonemal complex, REC-8/Rec8 and the MRN/X complex. PARG-1 shapes the recombination landscape and reinforces the tightly regulated control of crossover numbers without requiring its catalytic activity. We unravel roles in regulating meiosis, beyond its enzymatic activity in poly(ADP-ribose) catabolism. Poly(ADP-ribose) glycohydrolase (PARG) is involved in different cellular processes including DNA repair. Here the authors reveal a role for PARG in regulating meiotic DNA double strand break induction and repair in Caenorhabditis elegans.

Links

GA20-08819S, research and development project

Name: Pochopení úlohy PARG při podpoře tvorby a oprav dvouřetězcových zlomů DNA v meióze

Investor: Czech Science Foundation

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

JANISIW, Eva, Marilina RAICES, Fabiola BALMIR, Luis F. PAULIN, Antoine BAUDRIMONT, Arndt VON HAESELER, Judith L. YANOWITZ, Verena JANTSCH and Nicola SILVA. Poly(ADP-ribose) glycohydrolase coordinates meiotic DNA double-strand break induction and repair independent of its catalytic activity. Nature Communications. London: Nature Publishing Group, 2020, vol. 11, No 1, p. 1-15. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/s41467-020-18693-1.

@article{1685079,
   author = {Janisiw, Eva and Raices, Marilina and Balmir, Fabiola and Paulin, Luis F. and Baudrimont, Antoine and von Haeseler, Arndt and Yanowitz, Judith L. and Jantsch, Verena and Silva, Nicola},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41467-020-18693-1},
   keywords = {Poly(ADP-ribose) glycohydrolase; meiotic DNA double-strand break induction},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Poly(ADP-ribose) glycohydrolase coordinates meiotic DNA double-strand break induction and repair independent of its catalytic activity},
   url = {https://www.nature.com/articles/s41467-020-18693-1.pdf},
   volume = {11},
   year = {2020}
}

TY  - JOUR
ID  - 1685079
AU  - Janisiw, Eva - Raices, Marilina - Balmir, Fabiola - Paulin, Luis F. - Baudrimont, Antoine - von Haeseler, Arndt - Yanowitz, Judith L. - Jantsch, Verena - Silva, Nicola
PY  - 2020
TI  - Poly(ADP-ribose) glycohydrolase coordinates meiotic DNA double-strand break induction and repair independent of its catalytic activity
JF  - Nature Communications
VL  - 11
IS  - 1
SP  - 1-15
EP  - 1-15
PB  - Nature Publishing Group
SN  - 20411723
KW  - Poly(ADP-ribose) glycohydrolase
KW  - meiotic DNA double-strand break induction
UR  - https://www.nature.com/articles/s41467-020-18693-1.pdf
L2  - https://www.nature.com/articles/s41467-020-18693-1.pdf
N2  - Poly(ADP-ribosyl)ation is a reversible post-translational modification synthetized by ADP-ribose transferases and removed by poly(ADP-ribose) glycohydrolase (PARG), which plays important roles in DNA damage repair. While well-studied in somatic tissues, much less is known about poly(ADP-ribosyl)ation in the germline, where DNA double-strand breaks are introduced by a regulated program and repaired by crossover recombination to establish a tether between homologous chromosomes. The interaction between the parental chromosomes is facilitated by meiotic specific adaptation of the chromosome axes and cohesins, and reinforced by the synaptonemal complex. Here, we uncover an unexpected role for PARG in coordinating the induction of meiotic DNA breaks and their homologous recombination-mediated repair in Caenorhabditis elegans. PARG-1/PARG interacts with both axial and central elements of the synaptonemal complex, REC-8/Rec8 and the MRN/X complex. PARG-1 shapes the recombination landscape and reinforces the tightly regulated control of crossover numbers without requiring its catalytic activity. We unravel roles in regulating meiosis, beyond its enzymatic activity in poly(ADP-ribose) catabolism. Poly(ADP-ribose) glycohydrolase (PARG) is involved in different cellular processes including DNA repair. Here the authors reveal a role for PARG in regulating meiotic DNA double strand break induction and repair in Caenorhabditis elegans.
ER  -

JANISIW, Eva, Marilina RAICES, Fabiola BALMIR, Luis F. PAULIN, Antoine BAUDRIMONT, Arndt VON HAESELER, Judith L. YANOWITZ, Verena JANTSCH and Nicola SILVA. Poly(ADP-ribose) glycohydrolase coordinates meiotic DNA double-strand break induction and repair independent of its catalytic activity. \textit{Nature Communications}. London: Nature Publishing Group, 2020, vol.~11, No~1, p.~1-15. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-020-18693-1.

Detailed Information on Publication Record