J 2020

Trebonia kvetii gen. nov., sp. nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam. nov.

RAPOPORT, Daria, Marketa SAGOVA-MARECKOVA, Ivo SEDLÁČEK, Jan PROVAZNIK, Stanislava KRÁLOVÁ et. al.

Basic information

Original name

Trebonia kvetii gen. nov., sp. nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam. nov.

Authors

RAPOPORT, Daria, Marketa SAGOVA-MARECKOVA, Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution), Jan PROVAZNIK, Stanislava KRÁLOVÁ (703 Slovakia, belonging to the institution), Dinko PAVLINIC, Vladimir BENES and Jan KOPECKY (guarantor)

Edition

International Journal of Systematic and Evolutionary Microbiology, London, Microbiology Society, 2020, 1466-5026

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.747

RIV identification code

RIV/00216224:14310/20:00116663

Organization unit

Faculty of Science

DOI

UT WoS

000577279800033

Keywords in English

acidic soil; acidophilic and actinobacteria

Tags

Tags

International impact, Reviewed
Změněno: 4/12/2020 11:34, Mgr. Marie Šípková, DiS.

Abstract

V originále

A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales , class Actinomycetia . The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T. The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips. The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1 omega 9 and 10-methyl-C17 : 0. The fatty acid pattern differed from all patterns currently described for actinobacterial genera. The organism contained as major menaquinones MK9(H6) and MK9(H8), which differentiated it from other actinobacterial families. Polar lipids were composed of six unidentified glycolipids, an unidentified phosphoglycolipid, two unidentified phospholipids and two unidentified aminolipids. Whole-cell sugars contained galactose, xylose and arabinose as major components. The peptidoglycan type was A1 gamma meso-diaminopimelic acid. The genomic DNA G+C content was 69.7 mol%. The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp. nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam. nov.