Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection

SMRČKA, Filip and Přemysl LUBAL. Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection. Molecules. Basel: MDPI, 2020, vol. 25, No 18, p. 1-16. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules25184164.

Basic information

• Original name: Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection
• Authors: SMRČKA, Filip (203 Czech Republic, belonging to the institution) and Přemysl LUBAL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Molecules, Basel, MDPI, 2020, 1420-3049.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 4.411
• RIV identification code: RIV/00216224:14310/20:00116793
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.3390/molecules25184164
• UT WoS: 000580745000001
• Keywords (in Czech): Ln(III) komplexy; makrocyklické ligandy; Ln(III) luminescence ve VIS/NIR oblasti; antenový ligand; NAD(P)H stanovení; enzymová sonda
• Keywords in English: Ln(III) complexes; macrocyclic ligands; Ln(III) luminescence in VIS/NIR range; antenna ligand; NAD(P)H determination; enzyme probe
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Ln(III) complexes of macrocyclic ligands are used in medicinal chemistry, for example as contrast agents in MRI or radiopharmaceutical compounds, and in diagnostics using fluorescence imaging. This paper is devoted to a spectroscopic study of Ln(III) ternary complexes consisting of macrocyclic heptadentate DO3A and bidentate 3-isoquinolinate (IQCA) ligands. IQCA serves as an efficient antenna ligand, leading to a higher quantum yield and Stokes shift (250–350 nm for Eu, Tb, Sm, Dy in VIS region, 550–650 nm for Yb, Nd in NIR region). The shielding-quenching effect of NAD(P)H on the luminescence of the Ln(III) ternary complexes was investigated in detail and this phenomenon was utilized for the analytical determination of this compound. This general approach was verified through an enzymatic reaction during which the course of ethanol transformation catalyzed by alcohol-dehydrogenase (ADH) was followed by luminescence spectroscopy. This method can be utilized for selective and sensitive determination of ethanol concentration and/or ADH enzyme activity. This new analytical method can also be used for other enzyme systems coupled with NAD(P)H/NAD(P)+ redox pairs.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

• LTC20044, research and development project: Name: Thermodynamic and kinetic investigations of metal complexes of polydentate ligands for biomedical, analytical and industrial applications (Acronym: NECTAR_COST)

Investor: Ministry of Education, Youth and Sports of the CR, INTER-COST

• MUNI/A/1424/2019, interní kód MU: Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2020

Investor: Masaryk University, Category A

• 692298, interní kód MU: Name: MEDGENET - Medical genomics and epigenomics network (Acronym: MEDGENET)

Investor: European Union, Spreading excellence and widening participation