Cell type specific adhesion to surfaces functionalised by amine
plasma polymer

J 2020

Cell type specific adhesion to surfaces functionalised by amine plasma polymer

ČERNOCHOVÁ, Petra, Lucie BLAHOVÁ, Jiřina MEDALOVÁ, David NEČAS, Miroslav MICHLÍČEK et. al.

Basic information

Original name

Cell type specific adhesion to surfaces functionalised by amine plasma polymer

Authors

ČERNOCHOVÁ, Petra (203 Czech Republic, belonging to the institution), Lucie BLAHOVÁ (203 Czech Republic, belonging to the institution), Jiřina MEDALOVÁ (203 Czech Republic, belonging to the institution), David NEČAS (203 Czech Republic, belonging to the institution), Miroslav MICHLÍČEK (203 Czech Republic, belonging to the institution), Preeti KAUSHIK (356 India, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Jana BARTOŠÍKOVÁ (203 Czech Republic, belonging to the institution), Anton MANAKHOV (643 Russian Federation, belonging to the institution), Lucie BAČÁKOVÁ (203 Czech Republic) and Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific Reports, London, Nature Publishing Group, 2020, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10305 Fluids and plasma physics

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.379

RIV identification code

RIV/00216224:14310/20:00114429

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-020-65889-y

UT WoS

000543956100001

Keywords in English

Biomaterials - cells; Cell adhesion

Abstract

V originále

Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs.

Links

GA18-12774S, research and development project

Name: Plazmové polymery připravené na nanovlákenných membránách pro inženýrství cévní tkáně

Investor: Czech Science Foundation

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

ČERNOCHOVÁ, Petra, Lucie BLAHOVÁ, Jiřina MEDALOVÁ, David NEČAS, Miroslav MICHLÍČEK, Preeti KAUSHIK, Jan PŘIBYL, Jana BARTOŠÍKOVÁ, Anton MANAKHOV, Lucie BAČÁKOVÁ and Lenka ZAJÍČKOVÁ. Cell type specific adhesion to surfaces functionalised by amine plasma polymer. Scientific Reports. London: Nature Publishing Group, 2020, vol. 10, No 1, p. 1-14. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-65889-y.

@article{1689019,
   author = {Černochová, Petra and Blahová, Lucie and Medalová, Jiřina and Nečas, David and Michlíček, Miroslav and Kaushik, Preeti and Přibyl, Jan and Bartošíková, Jana and Manakhov, Anton and Bačáková, Lucie and Zajíčková, Lenka},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-020-65889-y},
   keywords = {Biomaterials - cells; Cell adhesion},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   note = {doc. Zajíčková - dle rozhodnutí ředitelů 5.3.2024},
   title = {Cell type specific adhesion to surfaces functionalised by amine plasma polymer},
   url = {https://www.nature.com/articles/s41598-020-65889-y},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1689019
AU  - Černochová, Petra - Blahová, Lucie - Medalová, Jiřina - Nečas, David - Michlíček, Miroslav - Kaushik, Preeti - Přibyl, Jan - Bartošíková, Jana - Manakhov, Anton - Bačáková, Lucie - Zajíčková, Lenka
PY  - 2020
TI  - Cell type specific adhesion to surfaces functionalised by amine plasma polymer
JF  - Scientific Reports
VL  - 10
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - Nature Publishing Group
SN  - 20452322
N1  - doc. Zajíčková - dle rozhodnutí ředitelů 5.3.2024
KW  - Biomaterials - cells
KW  - Cell adhesion
UR  - https://www.nature.com/articles/s41598-020-65889-y
L2  - https://www.nature.com/articles/s41598-020-65889-y
N2  - Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs.
ER  -

ČERNOCHOVÁ, Petra, Lucie BLAHOVÁ, Jiřina MEDALOVÁ, David NEČAS, Miroslav MICHLÍČEK, Preeti KAUSHIK, Jan PŘIBYL, Jana BARTOŠÍKOVÁ, Anton MANAKHOV, Lucie BAČÁKOVÁ and Lenka ZAJÍČKOVÁ. Cell type specific adhesion to surfaces functionalised by amine plasma polymer. \textit{Scientific Reports}. London: Nature Publishing Group, 2020, vol.~10, No~1, p.~1-14. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-65889-y.

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