a 2020

Near-infrared Polymethine Dyes for Targeted Biomarker Detection

PASTUCHA, Matěj, Martin POTRUSIL, Zdeněk FARKA and Peter ŠEBEJ

Basic information

Original name

Near-infrared Polymethine Dyes for Targeted Biomarker Detection

Authors

Edition

XXth Interdisciplinary Meeting of Young Researchers and Students in the Field of Chemistry, Biochemistry, Molecular Biology, and Biomaterials, 2020

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Organization unit

Faculty of Science

ISSN

Změněno: 9/11/2020 11:34, Mgr. Matěj Pastucha, Ph.D.

Abstract

V originále

Fluorescent dyes have a wide scope of use in life-sciences and medicine, most commonly as imaging agents, either alone, or in connection with antibodies in immunofluorescence experiments. Biological tissues absorb visible light and a tissue-transparent window (TTW) in the near-infrared (NIR) region is preferred for imaging purposes. This is particularly true in the fluorescence image-guided surgery. Polymethine dyes are good candidates for applications in the TTW and their properties can be tuned by changes in the polymethine chain length and substituents. We chose the indocyanine green (ICG) as an initial model as it is the only NIR dye certified by the FDA for use in clinical medicine. It is usually used as a dye with very limited selectivity, but when conjugated to an antibody, it can also be used for specific visualization of a biomarker of interest. We chose the carbohydrate antigen (CA) 19-9 as a promising target because it is already successfully used in the diagnosis and monitoring of treatment outcome in patients with pancreatic cancer. First, we labeled the mouse anti CA 19-9 antibody with the ICG NHS ester. The conjugate was successfully tested in a microtiter plate-based fluorescence immunoassay. Testing of the conjugate for detection of CA 19-9 in cell lines and tissue sections follows. We aim to develop a platform for assessing the performance of the newly synthesized polymethine fluorescent labels in complex biological systems.

Links

GJ20-30004Y, research and development project
Name: Nové strukturní motivy pro rozšíření možností lineárních polymethinových barviv
Investor: Czech Science Foundation
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR