| KOSTIV, Uliana, Zdeněk FARKA, Matthias Jürgen MICKERT, Hans-Heiner GORRIS, Nadiia VELYCHKIVSKA, Ognen POP-GEORGIEVSKI, Matěj PASTUCHA, Eliška ODSTRČILÍKOVÁ, Petr SKLÁDAL and Daniel HORÁK. Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications. Biomacromolecules. Washington, DC: American Chemical Society, 2020, vol. 21, No 11, p. 4502-4513. ISSN 1525-7797. Available from: https://dx.doi.org/10.1021/acs.biomac.0c00459. |
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@article{1690977,
author = {Kostiv, Uliana and Farka, Zdeněk and Mickert, Matthias Jürgen and Gorris, HansandHeiner and Velychkivska, Nadiia and PopandGeorgievski, Ognen and Pastucha, Matěj and Odstrčilíková, Eliška and Skládal, Petr and Horák, Daniel},
article_location = {Washington, DC},
article_number = {11},
doi = {http://dx.doi.org/10.1021/acs.biomac.0c00459},
keywords = {photon-upconversion nanoparticle; UCNP; bioconjugation; poly(ethylene glycol); click chemistry; upconversion-linked immunosorbent assay; ULISA; human serum albumin},
language = {eng},
issn = {1525-7797},
journal = {Biomacromolecules},
title = {Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications},
url = {https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459},
volume = {21},
year = {2020}
}
TY - JOUR
ID - 1690977
AU - Kostiv, Uliana - Farka, Zdeněk - Mickert, Matthias Jürgen - Gorris, Hans-Heiner - Velychkivska, Nadiia - Pop-Georgievski, Ognen - Pastucha, Matěj - Odstrčilíková, Eliška - Skládal, Petr - Horák, Daniel
PY - 2020
TI - Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications
JF - Biomacromolecules
VL - 21
IS - 11
SP - 4502-4513
EP - 4502-4513
PB - American Chemical Society
SN - 15257797
KW - photon-upconversion nanoparticle
KW - UCNP
KW - bioconjugation
KW - poly(ethylene glycol)
KW - click chemistry
KW - upconversion-linked immunosorbent assay
KW - ULISA
KW - human serum albumin
UR - https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459
L2 - https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459
N2 - Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis; however, they are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction; alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL).
ER -
KOSTIV, Uliana, Zdeněk FARKA, Matthias Jürgen MICKERT, Hans-Heiner GORRIS, Nadiia VELYCHKIVSKA, Ognen POP-GEORGIEVSKI, Matěj PASTUCHA, Eliška ODSTRČILÍKOVÁ, Petr SKLÁDAL and Daniel HORÁK. Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications. \textit{Biomacromolecules}. Washington, DC: American Chemical Society, 2020, vol.~21, No~11, p.~4502-4513. ISSN~1525-7797. Available from: https://dx.doi.org/10.1021/acs.biomac.0c00459.
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