J 2020

Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications

KOSTIV, Uliana, Zdeněk FARKA, Matthias Jürgen MICKERT, Hans-Heiner GORRIS, Nadiia VELYCHKIVSKA et. al.

Basic information

Original name

Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications

Authors

KOSTIV, Uliana (804 Ukraine), Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution), Matthias Jürgen MICKERT (276 Germany), Hans-Heiner GORRIS (276 Germany), Nadiia VELYCHKIVSKA (203 Czech Republic), Ognen POP-GEORGIEVSKI (203 Czech Republic), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Eliška ODSTRČILÍKOVÁ (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Daniel HORÁK (203 Czech Republic)

Edition

Biomacromolecules, Washington, DC, American Chemical Society, 2020, 1525-7797

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 6.988

RIV identification code

RIV/00216224:14310/20:00116881

Organization unit

Faculty of Science

DOI

UT WoS

000592221000010

Keywords in English

photon-upconversion nanoparticle; UCNP; bioconjugation; poly(ethylene glycol); click chemistry; upconversion-linked immunosorbent assay; ULISA; human serum albumin

Tags

Tags

International impact, Reviewed
Změněno: 22/1/2021 10:12, Mgr. Marie Šípková, DiS.

Abstract

V originále

Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis; however, they are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction; alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL).

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
LTAB19011, research and development project
Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel
Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION