Detailed Information on Publication Record
2020
Current density as routine parameter for description of ionic membrane current: is it always the best option?
KULA, Roman, Markéta BÉBAROVÁ, Peter MATEJOVIČ, Jiří ŠIMURDA, Michal PÁSEK et. al.Basic information
Original name
Current density as routine parameter for description of ionic membrane current: is it always the best option?
Authors
KULA, Roman (203 Czech Republic, belonging to the institution), Markéta BÉBAROVÁ (203 Czech Republic, guarantor, belonging to the institution), Peter MATEJOVIČ (203 Czech Republic, belonging to the institution), Jiří ŠIMURDA (203 Czech Republic, belonging to the institution) and Michal PÁSEK (203 Czech Republic, belonging to the institution)
Edition
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2020, 0079-6107
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.667
RIV identification code
RIV/00216224:14110/20:00118627
Organization unit
Faculty of Medicine
UT WoS
000582745400005
Keywords in English
Ionic current; Current density; Current-capacitance correlation; Current-capacitance proportionality; Rat cardiomyocyte; Normalisation by ratio
Tags
International impact, Reviewed
Změněno: 12/5/2021 14:14, Mgr. Tereza Miškechová
Abstract
V originále
The current density (J) is a parameter routinely used to characterize individual ionic membrane currents. Its evaluation is based on the presumption that the magnitude of whole-cell ionic membrane current (I) is directly proportional to the cell membrane capacitance (C), i.e. I positively and strongly correlates with C and the regression line describing I-C relation intersects the y-axis close to the origin of coordinates. We aimed to prove the presumption in several examples and find whether the conversion of I to J could be always beneficial. I-C relation was analysed in several potassium currents, measured in rat atrial myocytes (in inward rectifier currents, I-K1, and both the constitutively active and acetylcholine-induced components of acetylcholine-sensitive current, I-K(Ach)CONST and I-K(Ach)ACH), and in rat ventricular myocytes (transient outward current I-to). I-C correlation was estimated by the Pearson coefficient (r). A coefficient (k) was newly suggested describing deviation of the regression intercept from zero in currents with considerable r value. Based on mathematical simulations, I was satisfactorily proportional to C when r >= 0.6 and k <= 0.2 which was fulfilled in I-K1 and I-K(Ach)ACH (r = 0.84, k = 0.20, and r = 0.61, k = 0.06, respectively). IeC correlation was significantly positive, but weak in I-K(Ach)CONST (r = 0.42), and virtually missing in Ito (r = 0.04). The impaired I-C proportionality in I-K(Ach)CONST and Ito likely reflects heterogeneity of the channel expression. We conclude that the conversion of I to J should be avoided when I-C proportionality is absent. Otherwise, serious misinterpretation of data may arise. (C) 2019 Elsevier Ltd. All rights reserved.
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