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@article{1692357,
author = {Baloun, Jiří and Bencúrová, Petra and Totková, Tereza and Kubová, Hana and Hermanová, Markéta and Hendrych, Michal and Pail, Martin and Pospíšilová, Šárka and Brázdil, Milan},
article_location = {Lausanne},
article_number = {SEP},
doi = {http://dx.doi.org/10.3389/fnins.2020.00924},
keywords = {miRNA; mesial temporal lobe epilepsy; animal model; human; sequencing; cross comparison study},
language = {eng},
issn = {1662-453X},
journal = {Frontiers in Neuroscience},
title = {Epilepsy miRNA Profile Depends on the Age of Onset in Humans and Rats},
url = {https://www.frontiersin.org/articles/10.3389/fnins.2020.00924/full},
volume = {14},
year = {2020}
}
TY - JOUR
ID - 1692357
AU - Baloun, Jiří - Bencúrová, Petra - Totková, Tereza - Kubová, Hana - Hermanová, Markéta - Hendrych, Michal - Pail, Martin - Pospíšilová, Šárka - Brázdil, Milan
PY - 2020
TI - Epilepsy miRNA Profile Depends on the Age of Onset in Humans and Rats
JF - Frontiers in Neuroscience
VL - 14
IS - SEP
SP - 924
EP - 924
PB - Frontiers Media S.A.
SN - 1662453X
KW - miRNA
KW - mesial temporal lobe epilepsy
KW - animal model
KW - human
KW - sequencing
KW - cross comparison study
UR - https://www.frontiersin.org/articles/10.3389/fnins.2020.00924/full
L2 - https://www.frontiersin.org/articles/10.3389/fnins.2020.00924/full
N2 - Temporal lobe epilepsy (TLE) is a severe neurological disorder accompanied by recurrent spontaneous seizures. Although the knowledge of TLE onset is still incomplete, TLE pathogenesis most likely involves the aberrant expression of microRNAs (miRNAs). miRNAs play an essential role in organism homeostasis and are widely studied in TLE as potential therapeutics and biomarkers. However, many discrepancies in discovered miRNAs occur among TLE studies due to model-specific miRNA expression, different onset ages of epilepsy among patients, or technology-related bias. We employed a massive parallel sequencing approach to analyze brain tissues from 16 adult mesial TLE (mTLE)/hippocampal sclerosis (HS) patients, 8 controls and 20 rats with TLE-like syndrome, and 20 controls using the same workflow and categorized these subjects based on the age of epilepsy onset. All categories were compared to discover overlapping miRNAs with an aberrant expression, which could be involved in TLE. Our cross-comparative analyses showed distinct miRNA profiles across the age of epilepsy onset and found that the miRNA profile in rats with adult-onset TLE shows the closest resemblance to the profile in mTLE/HS patients. Additionally, this analysis revealed overlapping miRNAs between patients and the rat model, which should participate in epileptogenesis and ictogenesis. Among the overlapping miRNAs stand out miR-142-5p and miR-142-3p, which regulate immunomodulatory agents with pro-convulsive effects and suppress neuronal growth. Our cross-comparison study enhanced the insight into the effect of the age of epilepsy onset on miRNA expression and deepened the knowledge of epileptogenesis. We employed the same methodological workflow in both patients and the rat model, thus improving the reliability and accuracy of our results.
ER -
BALOUN, Jiří, Petra BENCÚROVÁ, Tereza TOTKOVÁ, Hana KUBOVÁ, Markéta HERMANOVÁ, Michal HENDRYCH, Martin PAIL, Šárka POSPÍŠILOVÁ a Milan BRÁZDIL. Epilepsy miRNA Profile Depends on the Age of Onset in Humans and Rats. Online. \textit{Frontiers in Neuroscience}. Lausanne: Frontiers Media S.A., 2020, roč.~14, SEP, s.~924-937. ISSN~1662-453X. Dostupné z: https://dx.doi.org/10.3389/fnins.2020.00924. [citováno 2024-04-24]
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