| SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Petr SKLÁDAL a Karel LACINA. Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing. ACS Applied Materials & Interfaces. Washington, D.C.: American Chemical Society, 2020, roč. 12, č. 12, s. 14620-14628. ISSN 1944-8244. Dostupné z: https://dx.doi.org/10.1021/acsami.0c02650. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1694637,
author = {Sopoušek, Jakub and Věžník, Jakub and Skládal, Petr and Lacina, Karel},
article_location = {Washington, D.C.},
article_number = {12},
doi = {http://dx.doi.org/10.1021/acsami.0c02650},
keywords = {nanopores; biosensing; electrochemical impedance spectroscopy; polystyrene nanoparticles; influence of charge},
language = {eng},
issn = {1944-8244},
journal = {ACS Applied Materials & Interfaces},
title = {Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing},
url = {https://doi.org/10.1021/acsami.0c02650},
volume = {12},
year = {2020}
}
TY - JOUR
ID - 1694637
AU - Sopoušek, Jakub - Věžník, Jakub - Skládal, Petr - Lacina, Karel
PY - 2020
TI - Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing
JF - ACS Applied Materials & Interfaces
VL - 12
IS - 12
SP - 14620-14628
EP - 14620-14628
PB - American Chemical Society
SN - 19448244
KW - nanopores
KW - biosensing
KW - electrochemical impedance spectroscopy
KW - polystyrene nanoparticles
KW - influence of charge
UR - https://doi.org/10.1021/acsami.0c02650
L2 - https://doi.org/10.1021/acsami.0c02650
N2 - Blockage of a nanopore by an analyte molecule has emerged as a promising concept for electrochemical biosensing. Nanoporous structures can be formed on the electrode surface simply by packing spherical nanoparticles in a dense planar arrangement. Modification of the nanoparticles with human serum albumin (HSA) and its interaction with the corresponding antibody (anti-HSA) can induce nanopore-blockage which significantly hinders permeation of the redox probe ([Fe(CN6)](4-/3-)). Interfaces of different parameters were studied using Electrochemical Impedance Spectroscopy (EIS), and counterintuitively, the influence of charge of the nanoparticles and other immobilized entities played a substantial role in the measurement. Our study reveals dominant effects including the presence of mixed output signal and resolves corresponding EIS biosensing-related challenges. Consequently, blocking the nanopores was introduced as an efficient technique which enables the application of EIS-based biosensing to real-world analytical issues.
ER -
SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Petr SKLÁDAL a Karel LACINA. Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing. \textit{ACS Applied Materials \&{} Interfaces}. Washington, D.C.: American Chemical Society, 2020, roč.~12, č.~12, s.~14620-14628. ISSN~1944-8244. Dostupné z: https://dx.doi.org/10.1021/acsami.0c02650.
|
