SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Petr SKLÁDAL and Karel LACINA. Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing. ACS Applied Materials & Interfaces. Washington, D.C.: American Chemical Society, 2020, vol. 12, No 12, p. 14620-14628. ISSN 1944-8244. doi:10.1021/acsami.0c02650.
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Basic information
Original name Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing
Authors SOPOUŠEK, Jakub (203 Czech Republic, belonging to the institution), Jakub VĚŽNÍK (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Karel LACINA (203 Czech Republic, guarantor, belonging to the institution).
Edition ACS Applied Materials & Interfaces, Washington, D.C. American Chemical Society, 2020, 1944-8244.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 9.229
RIV identification code RIV/00216224:14740/20:00114473
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1021/acsami.0c02650
UT WoS 000526552100106
Keywords in English nanopores; biosensing; electrochemical impedance spectroscopy; polystyrene nanoparticles; influence of charge
Tags CF NANO, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 22/2/2021 13:21.
Abstract
Blockage of a nanopore by an analyte molecule has emerged as a promising concept for electrochemical biosensing. Nanoporous structures can be formed on the electrode surface simply by packing spherical nanoparticles in a dense planar arrangement. Modification of the nanoparticles with human serum albumin (HSA) and its interaction with the corresponding antibody (anti-HSA) can induce nanopore-blockage which significantly hinders permeation of the redox probe ([Fe(CN6)](4-/3-)). Interfaces of different parameters were studied using Electrochemical Impedance Spectroscopy (EIS), and counterintuitively, the influence of charge of the nanoparticles and other immobilized entities played a substantial role in the measurement. Our study reveals dominant effects including the presence of mixed output signal and resolves corresponding EIS biosensing-related challenges. Consequently, blocking the nanopores was introduced as an efficient technique which enables the application of EIS-based biosensing to real-world analytical issues.
Links
GJ19-16273Y, research and development projectName: ROS-indukované uvolňování léčiv založené na interakcích ferocenylboronátů
Investor: Czech Science Foundation, ROS-Induced Drug Release Based on Interactions of Ferrocenylboronates
LM2015043, research and development projectName: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1252/2019, interní kód MUName: Podpora biochemického výzkumu v roce 2020
Investor: Masaryk University, Category A
MUNI/A/1359/2018, interní kód MUName: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019
Investor: Masaryk University, Category A
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