Variability in the Clearance of Lead Oxide Nanoparticles Is
Associated with Alteration of Specific Membrane Transporters

J 2020

Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters

DUMKOVÁ, Jana, Tereza SMUTNÁ, Lucie VRLÍKOVÁ, Hana KOTASOVÁ, Bohumil DOČEKAL et. al.

Basic information

Original name

Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters

Authors

DUMKOVÁ, Jana (203 Czech Republic, belonging to the institution), Tereza SMUTNÁ (203 Czech Republic, belonging to the institution), Lucie VRLÍKOVÁ, Hana KOTASOVÁ (203 Czech Republic, belonging to the institution), Bohumil DOČEKAL, Lukáš ČAPKA, Michaela KUCHYNKA (203 Czech Republic, belonging to the institution), Veronika JAKEŠOVÁ, Vendula PELKOVÁ (203 Czech Republic, belonging to the institution), Kamil KŘŮMAL, Pavel COUFALÍK, Pavel MIKUŠKA, Zbyněk VEČEŘA, Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Zuzana HUSÁKOVÁ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Marcela BUCHTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

ACS Nano, Washington, D.C. American Chemical Society, 2020, 1936-0851

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 15.881

RIV identification code

RIV/00216224:14310/20:00114474

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acsnano.9b08143

UT WoS

000526301400044

Keywords in English

clearance; nanoparticles; lead oxide; inhalation; ABC transporters; caveolins; LA-ICP-MS imaging

Abstract

V originále

Lead oxide nanoparticles (PbONPs), upon their entry into the lungs via inhalation, induce structural changes in primary and secondary target organs. The fate and ultrastructural localization of PbONPs in organs is known to be dependent on the specific organ. Here, we focused on the differences in the ability to clear the inhaled PbONPs from secondary target organs and on molecular and cellular mechanisms contributing to nanoparticle removal. Mice were exposed to PbONPs in whole-body inhalation chambers. Clearance of ionic lead and PbONPs (Pb/PbONPs) from the lungs and liver was very effective, with the lead being almost completely eliminated from the lungs and the physiological state of the lung tissue conspicuously restored. Kidneys exposed to nanoparticles did not exhibit serious signs of damage; however, LA-ICP-MS uncovered a certain amount of lead located preferentially in the kidney cortex even after a clearance period. The concentration of lead in femurs, as representatives of the axial skeleton, was the highest among studied organs at all designated time points after PbONP exposure, and the clearance ability of lead from the femurs was very low in contrast to other organs. The organ-specific increase of ABC transporters expression (ABCG2 in lungs and ABCC3 in the liver) was observed in exposed animals, suggesting their involvement in removing Pb/PbONPs from tissues. Moreover, the expression of caveolins and clathrin displayed a tissue-specific response to lead exposure. Our results uncovered high variability among the organs in their ability to clear Pb/PbONPs and in the transporters involved in this process.

Links

GA17-12774S, research and development project

Name: Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

Investor: Czech Science Foundation

GA20-02203S, research and development project

Name: Analýza tkáňové odpovědi na inhalaci nanočástic kovů a mechanismus jejich čištění

Investor: Czech Science Foundation

Citovat

DUMKOVÁ, Jana, Tereza SMUTNÁ, Lucie VRLÍKOVÁ, Hana KOTASOVÁ, Bohumil DOČEKAL, Lukáš ČAPKA, Michaela KUCHYNKA, Veronika JAKEŠOVÁ, Vendula PELKOVÁ, Kamil KŘŮMAL, Pavel COUFALÍK, Pavel MIKUŠKA, Zbyněk VEČEŘA, Tomáš VACULOVIČ, Zuzana HUSÁKOVÁ, Viktor KANICKÝ, Aleš HAMPL and Marcela BUCHTOVÁ. Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters. ACS Nano. Washington, D.C.: American Chemical Society, 2020, vol. 14, No 3, p. 3096-3120. ISSN 1936-0851. Available from: https://dx.doi.org/10.1021/acsnano.9b08143.

@article{1694656,
   author = {Dumková, Jana and Smutná, Tereza and Vrlíková, Lucie and Kotasová, Hana and Dočekal, Bohumil and Čapka, Lukáš and Kuchynka, Michaela and Jakešová, Veronika and Pelková, Vendula and Křůmal, Kamil and Coufalík, Pavel and Mikuška, Pavel and Večeřa, Zbyněk and Vaculovič, Tomáš and Husáková, Zuzana and Kanický, Viktor and Hampl, Aleš and Buchtová, Marcela},
   article_location = {Washington, D.C.},
   article_number = {3},
   doi = {http://dx.doi.org/10.1021/acsnano.9b08143},
   keywords = {clearance; nanoparticles; lead oxide; inhalation; ABC transporters; caveolins; LA-ICP-MS imaging},
   language = {eng},
   issn = {1936-0851},
   journal = {ACS Nano},
   title = {Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters},
   url = {https://doi.org/10.1021/acsnano.9b08143},
   volume = {14},
   year = {2020}
}

TY  - JOUR
ID  - 1694656
AU  - Dumková, Jana - Smutná, Tereza - Vrlíková, Lucie - Kotasová, Hana - Dočekal, Bohumil - Čapka, Lukáš - Kuchynka, Michaela - Jakešová, Veronika - Pelková, Vendula - Křůmal, Kamil - Coufalík, Pavel - Mikuška, Pavel - Večeřa, Zbyněk - Vaculovič, Tomáš - Husáková, Zuzana - Kanický, Viktor - Hampl, Aleš - Buchtová, Marcela
PY  - 2020
TI  - Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters
JF  - ACS Nano
VL  - 14
IS  - 3
SP  - 3096-3120
EP  - 3096-3120
PB  - American Chemical Society
SN  - 19360851
KW  - clearance
KW  - nanoparticles
KW  - lead oxide
KW  - inhalation
KW  - ABC transporters
KW  - caveolins
KW  - LA-ICP-MS imaging
UR  - https://doi.org/10.1021/acsnano.9b08143
L2  - https://doi.org/10.1021/acsnano.9b08143
N2  - Lead oxide nanoparticles (PbONPs), upon their entry into the lungs via inhalation, induce structural changes in primary and secondary target organs. The fate and ultrastructural localization of PbONPs in organs is known to be dependent on the specific organ. Here, we focused on the differences in the ability to clear the inhaled PbONPs from secondary target organs and on molecular and cellular mechanisms contributing to nanoparticle removal. Mice were exposed to PbONPs in whole-body inhalation chambers. Clearance of ionic lead and PbONPs (Pb/PbONPs) from the lungs and liver was very effective, with the lead being almost completely eliminated from the lungs and the physiological state of the lung tissue conspicuously restored. Kidneys exposed to nanoparticles did not exhibit serious signs of damage; however, LA-ICP-MS uncovered a certain amount of lead located preferentially in the kidney cortex even after a clearance period. The concentration of lead in femurs, as representatives of the axial skeleton, was the highest among studied organs at all designated time points after PbONP exposure, and the clearance ability of lead from the femurs was very low in contrast to other organs. The organ-specific increase of ABC transporters expression (ABCG2 in lungs and ABCC3 in the liver) was observed in exposed animals, suggesting their involvement in removing Pb/PbONPs from tissues. Moreover, the expression of caveolins and clathrin displayed a tissue-specific response to lead exposure. Our results uncovered high variability among the organs in their ability to clear Pb/PbONPs and in the transporters involved in this process.
ER  -

DUMKOVÁ, Jana, Tereza SMUTNÁ, Lucie VRLÍKOVÁ, Hana KOTASOVÁ, Bohumil DOČEKAL, Lukáš ČAPKA, Michaela KUCHYNKA, Veronika JAKEŠOVÁ, Vendula PELKOVÁ, Kamil KŘŮMAL, Pavel COUFALÍK, Pavel MIKUŠKA, Zbyněk VEČEŘA, Tomáš VACULOVIČ, Zuzana HUSÁKOVÁ, Viktor KANICKÝ, Aleš HAMPL and Marcela BUCHTOVÁ. Variability in the Clearance of Lead Oxide Nanoparticles Is Associated with Alteration of Specific Membrane Transporters. \textit{ACS Nano}. Washington, D.C.: American Chemical Society, 2020, vol.~14, No~3, p.~3096-3120. ISSN~1936-0851. Available from: https://dx.doi.org/10.1021/acsnano.9b08143.

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