Comparative Genomics Underlines Multiple Roles of Profftella,
an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins,
and Carotenoids.

J 2020

Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids.

NAKABACHI, Atsushi, Jörn PIEL, Igor MALENOVSKÝ and Yuu HIROSE

Basic information

Original name

Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids.

Authors

NAKABACHI, Atsushi (392 Japan), Jörn PIEL (756 Switzerland), Igor MALENOVSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Yuu HIROSE (392 Japan)

Edition

Genome biology and evolution, Oxford, Oxford University Press, 2020, 1759-6653

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.416

RIV identification code

RIV/00216224:14310/20:00116989

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1093/gbe/evaa175

UT WoS

000599652700006

Keywords in English

Diaphorin; defensive symbiont; diaphorin; hemolysin; reduced genome; secondary metabolite

Abstract

V originále

The Asian citrus psyllid Diaphorina citri (Insecta: Hemiptera: Psylloidea), a serious pest of citrus species worldwide, harbors vertically transmitted intracellular mutualists, Candidatus Profftella armatura (Profftella_DC, Gammaproteobacteria: Burkholderiales) and Candidatus Carsonella ruddii (Carsonella_DC, Gammaproteobacteria: Oceanospirillales). Large parts of the 460-kb genome of Profftella_DC are devoted to genes for synthesizing a polyketide toxin; diaphorin. To better understand the evolution of this unusual symbiont, the present study analyzed the genome of Profftella_Dco, a sister lineage to Profftella_DC, using Diaphorina cf. continua, a host psyllid congeneric with D. citri. The genome of coresiding Carsonella (Carsonella_Dco) was also analyzed. The analysis revealed nearly perfect synteny conservation in these genomes with their counterparts from D. citri.The substitution rate analysis further demonstrated genomic stability of Profftella which is comparable to that of Carsonella. Profftella_Dco and Profftella_DC shared all genes for the biosynthesis of diaphorin, hemolysin, riboflavin, biotin, and carotenoids, underlining multiple roles of Profftella, which may contribute to stabilizing symbiotic relationships with the host. However, acyl carrier proteins were extensively amplified in polyketide synthases DipP and DipT for diaphorin synthesis in Profftella_Dco. This level of acyl carrier protein augmentation, unprecedented in modular polyketide synthases of any known organism, is not thought to influence the polyketide structure but may improve the synthesis efficiency.

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Citovat

NAKABACHI, Atsushi, Jörn PIEL, Igor MALENOVSKÝ and Yuu HIROSE. Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids. Genome biology and evolution. Oxford: Oxford University Press, 2020, vol. 12, No 11, p. 1975-1987. ISSN 1759-6653. Available from: https://dx.doi.org/10.1093/gbe/evaa175.

@article{1695822,
   author = {Nakabachi, Atsushi and Piel, Jörn and Malenovský, Igor and Hirose, Yuu},
   article_location = {Oxford},
   article_number = {11},
   doi = {http://dx.doi.org/10.1093/gbe/evaa175},
   keywords = {Diaphorin; defensive symbiont; diaphorin; hemolysin; reduced genome; secondary metabolite},
   language = {eng},
   issn = {1759-6653},
   journal = {Genome biology and evolution},
   title = {Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids.},
   url = {https://doi.org/10.1093/gbe/evaa175},
   volume = {12},
   year = {2020}
}

TY  - JOUR
ID  - 1695822
AU  - Nakabachi, Atsushi - Piel, Jörn - Malenovský, Igor - Hirose, Yuu
PY  - 2020
TI  - Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids.
JF  - Genome biology and evolution
VL  - 12
IS  - 11
SP  - 1975-1987
EP  - 1975-1987
PB  - Oxford University Press
SN  - 17596653
KW  - Diaphorin
KW  - defensive symbiont
KW  - diaphorin
KW  - hemolysin
KW  - reduced genome
KW  - secondary metabolite
UR  - https://doi.org/10.1093/gbe/evaa175
L2  - https://doi.org/10.1093/gbe/evaa175
N2  - The Asian citrus psyllid Diaphorina citri (Insecta: Hemiptera: Psylloidea), a serious pest of citrus species worldwide, harbors vertically transmitted intracellular mutualists, Candidatus Profftella armatura (Profftella_DC, Gammaproteobacteria: Burkholderiales) and Candidatus Carsonella ruddii (Carsonella_DC, Gammaproteobacteria: Oceanospirillales). Large parts of the 460-kb genome of Profftella_DC are devoted to genes for synthesizing a polyketide toxin; diaphorin. To better understand the evolution of this unusual symbiont, the present study analyzed the genome of Profftella_Dco, a sister lineage to Profftella_DC, using Diaphorina cf. continua, a host psyllid congeneric with D. citri. The genome of coresiding Carsonella (Carsonella_Dco) was also analyzed. The analysis revealed nearly perfect synteny conservation in these genomes with their counterparts from D. citri.The substitution rate analysis further demonstrated genomic stability of Profftella which is comparable to that of Carsonella. Profftella_Dco and Profftella_DC shared all genes for the biosynthesis of diaphorin, hemolysin, riboflavin, biotin, and carotenoids, underlining multiple roles of Profftella, which may contribute to stabilizing symbiotic relationships with the host. However, acyl carrier proteins were extensively amplified in polyketide synthases DipP and DipT for diaphorin synthesis in Profftella_Dco. This level of acyl carrier protein augmentation, unprecedented in modular polyketide synthases of any known organism, is not thought to influence the polyketide structure but may improve the synthesis efficiency.
ER  -

NAKABACHI, Atsushi, Jörn PIEL, Igor MALENOVSKÝ and Yuu HIROSE. Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids. \textit{Genome biology and evolution}. Oxford: Oxford University Press, 2020, vol.~12, No~11, p.~1975-1987. ISSN~1759-6653. Available from: https://dx.doi.org/10.1093/gbe/evaa175.

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