NANOG/NANOGP8 Localizes at the Centrosome and is
Spatiotemporally Associated with Centriole Maturation

J 2020

NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation

MIKULENKOVÁ, Erika, Jakub NERADIL, Ondřej VYMAZAL, Jan ŠKODA, Renata VESELSKÁ et. al.

Basic information

Original name

NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation

Authors

MIKULENKOVÁ, Erika (203 Czech Republic, belonging to the institution), Jakub NERADIL (203 Czech Republic, belonging to the institution), Ondřej VYMAZAL (203 Czech Republic, belonging to the institution), Jan ŠKODA (203 Czech Republic, guarantor, belonging to the institution) and Renata VESELSKÁ (203 Czech Republic, belonging to the institution)

Edition

Cells, Basel, MDPI, 2020, 2073-4409

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.600

RIV identification code

RIV/00216224:14310/20:00116996

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/cells9030692

UT WoS

000529337400169

Keywords in English

NANOG; NANOGP8; human; localization; centrosome; mother centriole

Abstract

V originále

NANOG is a transcription factor involved in the regulation of pluripotency and stemness. The functional paralog of NANOG, NANOGP8, differs from NANOG in only three amino acids and exhibits similar reprogramming activity. Given the transcriptional regulatory role played by NANOG, the nuclear localization of NANOG/NANOGP8 has primarily been considered to date. In this study, we investigated the intriguing extranuclear localization of NANOG and demonstrated that a substantial pool of NANOG/NANOGP8 is localized at the centrosome. Using double immunofluorescence, the colocalization of NANOG protein with pericentrin was identified by two independent anti-NANOG antibodies among 11 tumor and non-tumor cell lines. The validity of these observations was confirmed by transient expression of GFP-tagged NANOG, which also colocalized with pericentrin. Mass spectrometry of the anti-NANOG immunoprecipitated samples verified the antibody specificity and revealed the expression of both NANOG and NANOGP8, which was further confirmed by real-time PCR. Using cell fractionation, we show that a considerable amount of NANOG protein is present in the cytoplasm of RD and NTERA-2 cells. Importantly, cytoplasmic NANOG was unevenly distributed at the centrosome pair during the cell cycle and colocalized with the distal region of the mother centriole, and its presence was markedly associated with centriole maturation. Along with the finding that the centrosomal localization of NANOG/NANOGP8 was detected in various tumor and non-tumor cell types, these results provide the first evidence suggesting a common centrosome-specific role of NANOG.

Links

MUNI/A/1127/2019, interní kód MU

Name: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 8 (Acronym: MBG 8)

Investor: Masaryk University, Category A

Citovat

MIKULENKOVÁ, Erika, Jakub NERADIL, Ondřej VYMAZAL, Jan ŠKODA and Renata VESELSKÁ. NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation. Cells. Basel: MDPI, 2020, vol. 9, No 3, p. 1-18. ISSN 2073-4409. Available from: https://dx.doi.org/10.3390/cells9030692.

@article{1696096,
   author = {Mikulenková, Erika and Neradil, Jakub and Vymazal, Ondřej and Škoda, Jan and Veselská, Renata},
   article_location = {Basel},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/cells9030692},
   keywords = {NANOG; NANOGP8; human; localization; centrosome; mother centriole},
   language = {eng},
   issn = {2073-4409},
   journal = {Cells},
   title = {NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation},
   url = {https://doi.org/10.3390/cells9030692},
   volume = {9},
   year = {2020}
}

TY  - JOUR
ID  - 1696096
AU  - Mikulenková, Erika - Neradil, Jakub - Vymazal, Ondřej - Škoda, Jan - Veselská, Renata
PY  - 2020
TI  - NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation
JF  - Cells
VL  - 9
IS  - 3
SP  - 1-18
EP  - 1-18
PB  - MDPI
SN  - 20734409
KW  - NANOG
KW  - NANOGP8
KW  - human
KW  - localization
KW  - centrosome
KW  - mother centriole
UR  - https://doi.org/10.3390/cells9030692
L2  - https://doi.org/10.3390/cells9030692
N2  - NANOG is a transcription factor involved in the regulation of pluripotency and stemness. The functional paralog of NANOG, NANOGP8, differs from NANOG in only three amino acids and exhibits similar reprogramming activity. Given the transcriptional regulatory role played by NANOG, the nuclear localization of NANOG/NANOGP8 has primarily been considered to date. In this study, we investigated the intriguing extranuclear localization of NANOG and demonstrated that a substantial pool of NANOG/NANOGP8 is localized at the centrosome. Using double immunofluorescence, the colocalization of NANOG protein with pericentrin was identified by two independent anti-NANOG antibodies among 11 tumor and non-tumor cell lines. The validity of these observations was confirmed by transient expression of GFP-tagged NANOG, which also colocalized with pericentrin. Mass spectrometry of the anti-NANOG immunoprecipitated samples verified the antibody specificity and revealed the expression of both NANOG and NANOGP8, which was further confirmed by real-time PCR. Using cell fractionation, we show that a considerable amount of NANOG protein is present in the cytoplasm of RD and NTERA-2 cells. Importantly, cytoplasmic NANOG was unevenly distributed at the centrosome pair during the cell cycle and colocalized with the distal region of the mother centriole, and its presence was markedly associated with centriole maturation. Along with the finding that the centrosomal localization of NANOG/NANOGP8 was detected in various tumor and non-tumor cell types, these results provide the first evidence suggesting a common centrosome-specific role of NANOG.
ER  -

MIKULENKOVÁ, Erika, Jakub NERADIL, Ondřej VYMAZAL, Jan ŠKODA and Renata VESELSKÁ. NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation. \textit{Cells}. Basel: MDPI, 2020, vol.~9, No~3, p.~1-18. ISSN~2073-4409. Available from: https://dx.doi.org/10.3390/cells9030692.

Detailed Information on Publication Record