VAŠÍČEK, Ondřej, Antonín LOJEK and Milan ČÍŽ. Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation. Journal of Physiology and Biochemistry. Dordrecht: Springer, 2020, vol. 76, No 1, p. 49-60. ISSN 1138-7548. Available from: https://dx.doi.org/10.1007/s13105-019-00714-3.
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Basic information
Original name Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation
Authors VAŠÍČEK, Ondřej, Antonín LOJEK and Milan ČÍŽ (203 Czech Republic, guarantor, belonging to the institution).
Edition Journal of Physiology and Biochemistry, Dordrecht, Springer, 2020, 1138-7548.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10608 Biochemistry and molecular biology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.158
RIV identification code RIV/00216224:14310/20:00117037
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s13105-019-00714-3
UT WoS 000520009600004
Keywords in English Serotonin; N-acetylserotonin; Melatonin; RAW264; 7 macrophages; Reactive oxygen species; Nitric oxide; Cytokines
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 23/11/2020 09:42.
Abstract
In this study, we focused on comparing the effects of serotonin and its metabolites on the functions of RAW264.7 cells (emphasis on oxidative burst and production of nitric oxide and cytokines), thereby expanding the scope of existing knowledge with advent of novel findings in this field. Changes in production of reactive oxygen species (ROS) by RAW264.7 cells after treatment with serotonin, N-acetylserotonin and melatonin were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all respective compounds were measured using TRAP and amperometrical method. Nitric oxide (NO) production was measured by Griess reagent and inducible NO synthase (iNOS) expression by Western blot. Cytokine production was assessed using the Mouse Cytokine Panel A Array kit and ELISA. We showed that all tested compounds were able to reduce oxidative stress, as well as inhibit production of inflammatory cytokines by macrophages. Of the tested compounds, serotonin and N-acetylserotonin were markedly better antioxidants than melatonin. In comparison, other effects of tested compounds were very similar. It can be concluded that antioxidant capacity of tested compounds is a major advantage in the early stages of inflammation. Since plasma concentrations of N-acetylserotonin and melatonin are lower than serotonin, it can be deduced that serotonin plays a key role in modulation of inflammation and the regulatory functions of immune cells, while also protecting cells against oxidative stress.
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