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@article{1698416,
author = {Dumková, Jana and Smutná, Tereza and Vrlíková, Lucie and Dočekal, Bohumil and Kristeková, Daniela and Večeřa, Zbyněk and Husáková, Zuzana and Jakešová, Veronika and Jedličková, Adriena and Mikuška, Pavel and Alexa, Lukáš and Coufalík, Pavel and Tvrdoňová, Michaela and Křůmal, Kamil and Vaculovič, Tomáš and Kanický, Viktor and Hampl, Aleš and Buchtová, Marcela},
article_location = {Basel},
article_number = {22},
doi = {http://dx.doi.org/10.3390/ijms21228738},
keywords = {clearance; lead nanoparticles; inhalation; LA-ICP-MS imaging; toxicity},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response},
url = {https://www.mdpi.com/1422-0067/21/22/8738},
volume = {21},
year = {2020}
}
TY - JOUR
ID - 1698416
AU - Dumková, Jana - Smutná, Tereza - Vrlíková, Lucie - Dočekal, Bohumil - Kristeková, Daniela - Večeřa, Zbyněk - Husáková, Zuzana - Jakešová, Veronika - Jedličková, Adriena - Mikuška, Pavel - Alexa, Lukáš - Coufalík, Pavel - Tvrdoňová, Michaela - Křůmal, Kamil - Vaculovič, Tomáš - Kanický, Viktor - Hampl, Aleš - Buchtová, Marcela
PY - 2020
TI - A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response
JF - International Journal of Molecular Sciences
VL - 21
IS - 22
SP - 1-27
EP - 1-27
PB - MDPI
SN - 14220067
KW - clearance
KW - lead nanoparticles
KW - inhalation
KW - LA-ICP-MS imaging
KW - toxicity
UR - https://www.mdpi.com/1422-0067/21/22/8738
L2 - https://www.mdpi.com/1422-0067/21/22/8738
N2 - The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO3)(2) NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO3)(2) NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO3)(2) NP inhalation; conversely, abundance of alpha-smooth muscle actin (alpha-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor kappa B (NF-kappa B) and selected cytokines, such as tumor necrosis factor alpha (TNF alpha), transforming growth factor beta 1 (TGF beta 1), interleukin 6(IL-6), IL-1 alpha and IL-1 beta , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO3)(2) NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period.
ER -
DUMKOVÁ, Jana, Tereza SMUTNÁ, Lucie VRLÍKOVÁ, Bohumil DOČEKAL, Daniela KRISTEKOVÁ, Zbyněk VEČEŘA, Zuzana HUSÁKOVÁ, Veronika JAKEŠOVÁ, Adriena JEDLIČKOVÁ, Pavel MIKUŠKA, Lukáš ALEXA, Pavel COUFALÍK, Michaela TVRDOŇOVÁ, Kamil KŘŮMAL, Tomáš VACULOVIČ, Viktor KANICKÝ, Aleš HAMPL and Marcela BUCHTOVÁ. A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, vol.~21, No~22, p.~1-27. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21228738.
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